Synergism of leukemic blast growth factors in medium conditioned by human bladder carcinoma cell line 5637
Asano, Y.; Shibuya, T.; Okamura, S.; Morioka, E.; Taniguchi, S.; Niho, Y.
International Journal of Cell Cloning 5(6): 504-510
ISSN/ISBN: 0737-1454 PMID: 3480913 DOI: 10.1002/stem.5530050608
Leukemic blast growth factors (LBGFs) are necessary for in vitro growth of clonogenic cells from patients with acute myeloblastic leukemia. As the human bladder carcinoma cell line 5637 had previously been reported to secrete abundant LBGFs into the culture supernatant, the LBGFs in 5637-conditioned medium (5637-CM) were characterized. Measurement of LBGFs was done using an in vitro leukemic blast colony assay in methylcellulose culture. LBGFs in 5637-CM were fractionated by anion exchange chromatography, and two peaks of activity were recovered. Pool B (high-salt eluent) and/or purified granulocyte colony-stimulating factor (G-CSF) were added to the clonogenic leukemic blast cell assays. It was found that pool B was more active than G-CSF in the majority of cases examined and that the two types of activity were synergistic in some cases.