EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Syntheses and modulations in the chromatin contents of histones h 1 o and h 1 during g 1 and s phases in chinese hamster cells



Syntheses and modulations in the chromatin contents of histones h 1 o and h 1 during g 1 and s phases in chinese hamster cells



Biochemistry 21(17): 3991-4001



Flow cytometry, conventional autoradiography and autoradiography employing high concentrations of high specific activity [3H]thymidine indicate that treatment of Chinese hamster ovary (line CHO) cells with butyrate truly blocks cells in G1 and cells blocked in G1 by Ile deprivation remain blocked in G1 when they are released into complete medium containing butyrate. Measurements of H1o content relative to core histones and H1o:H1 ratios indicate that H1o is enchanced somewhat in G1 cells arrested by Ile deprivation; however, treatment with butyrate greatly increases the H1o content in G1-blocked cells, and the enhancement is very sensitive to butyrate concentration. Measurements of relative histone contents in the isolated chromatin of synchronized cultures also suggest that the acid-soluble content of histone H1 (relative to core histones) becomes greatly depleted in the isolated chromatin when synchronized cells are blocked in early S phase by sequential use of Ile deprivation and hydroxyurea blockade. [3H]Lys incorporation, various protein ratios, and relative rates of deposition of newly synthesized H1o, H1 and H4 onto chromatin were measured during G1 and S in the absence of butyrate. H1o is synthesized and deposited onto chromatin during traverse of G1 and S phases so that its specific activity during G1 is 50-60% of its maximum value in S phase enriched (60-70%) cultures. The ratio between rates of deposition of new histones H1o and H1 onto chromatin reaches a maximum during G1 at 1.5-2.0 h after cells are released from the G1 block, and it declines about 6-fold as cells enter S phase. The H1o:H1 molar ratio is modulated in the isolated chromatin of synchronized cultures so that it reaches a maximum near the G1-S boundary. A dynamic picture of chromatin organization is suggested in which newly synthesized histone H1o binds to chromatin during traverse of G1 and S phases and histone H1 dissociates from (or becomes loosely bound to) chromatin during prolonged early S-phase block with hydroxyurea.

(PDF 0-2 workdays service: $29.90)

Accession: 006559465

Download citation: RISBibTeXText



Related references

Syntheses and modulations in the chromatin contents of histones H1 degrees and H1 during G1 and S phases in Chinese hamster cells. Biochemistry 21(17): 3991-4001, 1982

Histones of fractionated metaphase chromosomes of chinese hamster cells. Federation Proceedings 29(2): 730, 1970

The suppression by protein synthesis inhibitors of sister chromatid exchanges induced by exogenous agents in the eu chromatin and hetero chromatin of chinese hamster cells. Cell Structure & Function 6(3): 209-216, 1981

The distribution of sister chromatid exchanges and chromosomal aberrations induced by 5 fluorodeoxy uridine and ethyl methanesulfonate in the eu chromatin and hetero chromatin of chinese hamster cells. Cell Structure & Function 5(2): 175-182, 1980

Histones from Chinese hamster ovary cells. Multiple modified forms: a quantitative approach. European Journal of Biochemistry 135(1): 143-149, 1983

Biochemical and cytogenetical characterization of chinese hamster ovary x ray sensitive mutant cells xrs 5 and xrs 6 vi. the correlation between uv induced dna lesions and chromosomal aberrations and their modulations with inhibitors of dna repair synthesis. Mutation Research 235(2): 129-136, 1990

Histones of nucleolar-associated chromatin from hamster liver. General Physiology and Biophysics 8(2): 177-181, 1989

Ultrastructural characteristics of chinese hamster cells in different phases of the cell cycle. Journal of Cell Biology 75(2 PART 2): 7A, 1977

Analysis of chromatin structure in nickel silenced G12 Chinese hamster cells. Toxicological Sciences 72(S-1): 216, March, 2003

Histone phosphorylation and chromatin structure during mitosis in Chinese hamster cells. European Journal of Biochemistry 84(1): 1-15, 1978

Hamster female protein binding to chromatin, histones and DNA. Molecular Immunology 29(7-8): 837-845, 1992

Radiosensitization with 5-bromodeoxyuridine of Chinese hamster cells x-irradiated during different phases of the cell cycle. Radiation Research 47(3): 672-688, 1971

Chromosomal aberrations induced by tritiated thymidine during the S and G2 phases of Chinese hamster cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine 12(6): 597-600, 1967

Messenger rna synthesis during different phases of the cell cycle of cho chinese hamster ovary cells. Experientia (Basel) 32(6): 806, 1976