+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Synthesis and characterization of a fluorescence probe of the phase transition and dynamic properties of membranes

Synthesis and characterization of a fluorescence probe of the phase transition and dynamic properties of membranes

Biochemistry 22(25): 5714-5722

The synthesis and characterization of a new fluorescence probe whose emission spectra, anisotropies and wavelength-dependent decay times are highly sensitive to the phase state of phospholipid vesicles is described. This probe is 6-palmitoyl-2-[[2-(trimethylammonio)ethyl]methylamino]naphthalene chloride (Patman). The emission maximum of Patman shifts from 425 to 470 nm at the bilayer transition temperatures. The spectral properties of Patman reveal nanosecond time-dependent spectral shifts, which are the result of membrane relaxation around the excited state of Patman. The apparent fluorescence lifetimes of Patman are strongly dependent upon the emission wavelength, and the fluorescence phase and modulation data prove that the spectral shifts are due to an excited-state process, and not ground-state heterogeneity. As expected, the fluorescence anisotropies reflect the phase transitions of the bilayers. In addition, the anisotropies are dependent upon the emission wavelength because the duration of the excited state varies across the emission spectrum. The different apparent lifetimes across the emission spectrum allow the relaxed and unrelaxed emission spectra to be resolved by phase-sensitive detection of fluorescence. Also, the emission spectra of Patman show marked shifts to longer wavelengths as the excitation wavelength is increased. These red-edge excitation shifts are sensitive to the temperature and phase state of the bilayers.

(PDF 0-2 workdays service: $29.90)

Accession: 006561697

Download citation: RISBibTeXText

Related references

Synthesis and characterization of a fluorescence probe of the transition and dynamic properties of membranes. Biochemistry 22(25): 5714-5722, 1983

Dynamic properties of the haptenic site of lipid haptens in phosphatidylcholine membranes. Their relation to the phase transition of the host lattice. Biophysical Journal 52(2): 187-197, 1987

Transition metal(II) complexes of a novel symmetrical benzothiazole-based ligand: synthesis, spectral/structural characterization and fluorescence properties. Journal of Fluorescence 24(6): 1679-1686, 2015

Detection of a phase transition in red cell membranes using positronium as a probe. Biochimica et Biophysica Acta 646(2): 356-359, 1981

Synthesis, characterization, DNA binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin-2-hydroxy benzoyl hydrazone. Journal of Fluorescence 20(4): 891-905, 2011

4 tri methylaminophenyl 6 phenyl hexa 1 3 5 triene synthesis fluorescence properties and use as a fluorescence probe of lipid bi layers. Biochemistry: 7333-7338, 1981

1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene: synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers. Biochemistry 20(26): 7333-7338, 1981

Cholesterol-induced changes in hippocampal membranes utilizing a phase-sensitive fluorescence probe. Biochimica et Biophysica Acta 1848(8): 1699-1705, 2015

Intra molecular excimer fluorescence a new probe of phase transitions in synthetic phospho lipid membranes. Photochemistry & Photobiology 31(6): 539-546, 1980

Spectroscopic characterization of 2-amino-N-hexadecyl-benzamide (AHBA), a new fluorescence probe for membranes. Biophysical Chemistry 124(2): 125-133, 2006

Fluorescence properties of 1-heptanoylpyrene: a probe for hydrogen bonding in microaggregates and biological membranes. Journal of Photochemistry and Photobiology A: Chemistry 125(1-3): 29-38, 1999

Properties of bilayer membranes in the phase transition or phase separation region. Biochimica et Biophysica Acta 557(1): 24-31, 1979

Dynamic fluorescence measurements on the main phase transition of dipalmytoylphosphatidylcholine vesicles. European Biophysics Journal 13(6): 323-330, 1986

Red-edge excitation of fluorescence and dynamic properties of proteins and membranes. Biochemistry 23(13): 3013-3021, 1984

Detecting the phase transition in thylakoid membranes of maize inbred lines by means of delayed fluorescence. Plant Physiology and Biochemistry 81: 208-211, 2015