Synthesis and purification of the anti viral agent 1 2 deoxy 2 fluoro beta d arabinofuranosyl 5 iodocytosine labeled with iodine 125
Perlman, M.E.; Conti, P.S.; Schmall, B.; Watanabe, K.A.
International Journal of Nuclear Medicine and Biology 11(3-4): 215-218
ISSN/ISBN: 0047-0740 Accession: 006563400
The 125I labeled analog of 1-(2-deoxy-2-fluoro-.beta.-D-arabinofuranosyl)-5-iodocytosine (FIAC) was prepared for studies on the imaging of herpes simpex virus (HSV) encephalitis infections in animals [rats, as a model for human encephalitis]. Iodination of 1-(2-deoxy-2-fluoro-.beta.-D-arabinofuranosyl)cytosine.cntdot.HCl (FAC.cntdot.HCl) with Na 125I and NaI/HIO3 in aqueous acetic acid, and subsequent removal of residual iodine by CCl4 extraction and ion exchange chromatography, yielded [125I]FIAC in aqueous solution with a specific activity of 45.5 mCi/mmol in 44% radiochemical yield, and with a radiopurity of 96-97%. Methods for further purification are described.