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Synthetic activities of mass cultures and clones of human gingival fibroblasts



Synthetic activities of mass cultures and clones of human gingival fibroblasts



Experientia 42(1): 66-69



The percentage of synthesis dedicated to collagen is elevated in low-density cultures of human gingival fibroblasts, as is per-cell total protein synthetic activity and glycosaminoglycan accumulation. These observations can be explained, in part, by a decrease in membrane transport of precursor substance in high-density cultures. Synthetic activity by human fibroblasts can be reliably assayed in vitro using as few as 500 cells sparsely seeded. Such low-cell number assay is essential for study of single-cell clones, where replicative life span is limited.

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Accession: 006572670

Download citation: RISBibTeXText

PMID: 3943601

DOI: 10.1007/bf01975899


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