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Temperature sensitive mutants of influenza a udorn 72 h 3n 2 virus 1. isolation of temperature sensitive mutants some of which exhibit host dependent temperature sensitivity



Temperature sensitive mutants of influenza a udorn 72 h 3n 2 virus 1. isolation of temperature sensitive mutants some of which exhibit host dependent temperature sensitivity



Virology 117(1): 38-44



Influenza A/Udorn/72 virus was mutagenized to induce temperature-sensitive (ts) and host range mutations. The mutagenized virus was tested on both rhesus monkey kidney (RMK) and Madin-Darby canine kidney (MDCK) tissue culture at permissive and restrictive temperatures. Of 136 ts mutants isolated from 6185 clones tested, 83 were ts on RMK tissue and 133 were ts on MDCK. Each clone replicated at 34.degree. C on both RMK and MCDK tissue and thus failed to exhibit a difference in host range in these 2 tissues at permissive temperature. However, 32 clones were .gtoreq. 1000-fold restricted in plaque formation on MDCK cultures compared to RMK culture at 38.degree., 39.degree. and/or 40.degree. C. Four clones were more restricted on RMK cultures. These clones represent temperature-dependent host range (td-hr) mutants. The ts and td-hr mutants may be useful in exploring certain biological, genetic, biochemical and pathogenic properties of influenza A virus.

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Accession: 006585454

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PMID: 7064353


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