EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Testing for basic drugs in biological fluids by solvent extraction and dual capillary gas chromatography nitrogen phosphorus detector



Testing for basic drugs in biological fluids by solvent extraction and dual capillary gas chromatography nitrogen phosphorus detector



Journal of Analytical Toxicology 10(1): 10-14



Basic drugs were extracted from 1 mL of serum, urine, or other biological fluids under alkaline conditions into hexane:isoamyl alcohol (98:2). The drugs were back-extracted into acid and re-extracted into 50 .mu.L of chloroform:isoamyl alcohol (95:5) after the acid had been alkalinized. An aliquot of the chloroform:alcohol was injected onto a temperature programmed gas chromatograph equipped with two nitrogen phosphorus detectors and two fused silica capillary columns fitted into single injector. Results obtained from 388 serum samples (from suspected overdose patients) tested using this procedure are presented. The same extraction applied to a variety of body fluids proved reliable for quantitations of several of the basic drugs when a packed column was used.

(PDF emailed within 1 workday: $29.90)

Accession: 006590583

Download citation: RISBibTeXText



Related references

Testing for basic drugs in biological fluids by solvent extraction and dual capillary GC/NPD. Journal of Analytical Toxicology 10(1): 10-14, 1986

Screening of basic drugs in biological samples using dual column capillary chromatography and nitrogen-phosphorus detectors. Journal of Analytical Toxicology 10(5): 198-204, 1986

Determination of basic drugs extracted from biological matrices by means of solid-phase extraction and wide-bore capillary gas chromatography with nitrogen-phosphorus detection. Journal of Analytical Toxicology 18(3): 150-153, 1994

Determination of fluconazole in biological fluids by capillary column gas chromatography with a nitrogen detector. Journal of Pharmaceutical Sciences 77(6): 534-535, 1988

Determination of amino acids in biological fluids by capillary gas chromatography with nitrogen-phosphorus selective detection. Journal of Pharmaceutical & Biomedical Analysis 15(9-10): 1271-1279, 1997

A new rapid gas chromatography method for the detection of basic drugs in post mortem blood using a nitrogen phosphorus detector 2 quantitative analysis. Journal of Analytical Toxicology 3(4): 155-157, 1979

Screening and quantification of hypnotic sedatives in serum by capillary gas chromatography with a nitrogen-phosphorus detector, and confirmation by capillary gas chromatography-mass spectrometry. Clinical Chemistry 32(2): 325-328, 1986

A new rapid gas chromatography method for the detection of basic drugs in post mortem blood using a nitrogen phosphorus detector part 1 qualitative analysis. Journal of Analytical Toxicology 2(1): 26-31, 1978

Screening for neutral and basic drugs in blood by dual fused-silica column chromatography with nitrogen-phosphorus detection. Clinical Chemistry 34(7): 1492-1497, 1988

Detection of alprazolam (Xanax) and its metabolites in urine using dual capillary column, dual nitrogen detector gas chromatography. Journal of Analytical Toxicology 11(6): 247-251, 1987

Determination of diphenhydramine in biological fluids by capillary gas chromatography using nitrogen-phosphorus detection. Application to placental transfer studies in pregnant sheep. Journal of Chromatography 378(2): 385-393, 1986

A retention index library for commonly encountered drugs and metabolites using tri-n-alkylamines as reference compounds, nitrogen-phosphorus detectors, and dual capillary chromatography. Journal of Analytical Toxicology 11(5): 210-214, 1987

Determination of several psychiatric drugs in whole blood using capillary gas-liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures. Forensic Science International 155(2-3): 193-204, 2005

Determination of basic drugs in post mortem tissues a micro extraction technique utilizing gas liquid chromatography nitrogen phosphorus detectors of effluents. Journal of Analytical Toxicology 6(4): 181-184, 1982

Development of a standardized analysis strategy for basic drugs using ion-pair extraction and high-performance liquid chromatography. VIII. Method construction for the determination of mebeverine in tablets and biological fluids. Journal of Chromatography 377: 391-398, 1986