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The absorption fluorescence and phosphorescence of phalloidin and alpha amanitin


The absorption fluorescence and phosphorescence of phalloidin and alpha amanitin



Biochimica et Biophysica Acta 760(3): 411-414



ISSN/ISBN: 0005-2728

The peptides, phalloidin and .alpha.-amanitin, contain 2 unusual indole-derived chromophores, whose absorption, fluorescence and phosphocrescence in aqueous solutions were described. Fluorescence was weak, but phosphorescence at 77.degree. K was intense. Phalloidin underwent adiabatic photodissociation in alkaline solutions and fluorescenced from the indole anion form. The phenolic or the indolic protons of .alpha.-amanitin did not undergo photodissociation. The pKa value of the phenolic hydroxy group of .alpha.-amanitin was 9.71.degree. at 23.degree. C.

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