+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The action of procainamide and quinidine on the alpha 1-receptor-operated channels in smooth muscle cells of guinea-pig taenia caeci



The action of procainamide and quinidine on the alpha 1-receptor-operated channels in smooth muscle cells of guinea-pig taenia caeci



European Journal of Pharmacology 137(2-3): 233-240



The effect of procainamide (2.0-5.0 mM) and quinidine (0.2-1.0 mM) on the .alpha.1 response evoked by adrenaline (3 .times. 10-6 M) in smooth muscle cells of guinea-pig taenia caeci (22.degree. C) was studied in the presence of yohimbine (3 .times. 10-6 M), propranolol (3 .times. 10-6 M) or atropine (10-6 M). The electrotonic potential elicited by the application of a constant current to the preparation was slightly increased (about 10%) by procainamide (5.0 mM) but not by quinidine (1.0 mM). The double-sucrose gap method was used for measurements. The .alpha.1 response evoked by adrenaline in the absence of extracellular calcium (15 min) was represented by a transient hyperpolarization of the muscle cells, while the hyperpolarization elicited in the presence of calcium was sustained. The hyperpolarization is caused by enhancement of the potassium efflux assumed to be linked with mobilization of calcium form a cellular structure. Superfusion of the preparation with calcium-containing solution to replenish the calcium store in the presence of procainamide (10 min) before the .alpha.1 response evoked in the absence of calcium and procainamide did not affect the transient hyperpolarization. Quinidine, however, suppressed the .alpha.1 response when the same procedure was followed. Both the transient and the sustained hyperpolarization evoked in smooth muscle cells in the presence of procainamide (15 min) or quinidine in calcium-containing or in calcium-free solution, respectively, were inhibited. The .alpha.1 response was reflected by a depolarization of the muscle cells after the potassium channels had been blocked with apamin (3 .times. 10-7 M, 20 min). The transient depolarization observed after .alpha.1-receptor stimulation under calcium-free conditions was also suppressed by procainamide and quinidine. These results can be explained by assuming that calcium mobilization, activated after stimulation of the .alpha.1-receptors, is inhibited by procainamide and quinidine. A possible additional effect of quinidine on replenishment of the calcium store from the extracellular space is not excluded.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006595979

Download citation: RISBibTeXText

PMID: 2440706


Related references

Modification of allo graft survival in rats by blood transfusion to the donor. Transplantation (Baltimore) 36(4): 362-365, 1983

Hypoxia-induced inhibition of adenosine kinase potentiates cardiac adenosine release. Circulation Research 81(2): 154-164, 1997

Sodium conductance in calcium channels of guinea-pig ventricular cells induced by removal of external calcium ions. Pflugers Archiv 407(5): 465-475, 1986

Saturation of calcium channels and surface charge effects in skeletal muscle fibres of the frog. Journal of Physiology 351: 135-154, 1984

The cell shedding rate of the rabbit corneal epithelium. Investigative Ophthalmology and Visual Science 36(4): S696, 1995

Changes in membrane potential and membrane fluidity in tetrahymena pyriformis in association with chemo reception of hydrophobic stimuli fluorescence studies. Biochemistry 19(23): 5339-5344, 1980

The multiphase kinetics of phosphate uptake by plant roots. Journal of Radioanalytical and Nuclear Chemistry 95(4): 263-268, 1985

Some effects of organic anions on excitability and excitation-contraction coupling in frog skeletal muscle. Canadian Journal of Physiology and Pharmacology 55(3): 700-708, 1977

Effect of cromakalim on contractions in rabbit isolated renal artery in the presence and absence of extracellular calcium. British Journal of Pharmacology 98(4): 1303-1311, 1989

The effect of cisapride on gastro-oesophageal dysfunction in systemic sclerosis: a controlled manometric study. British Journal of Clinical Pharmacology 31(6): 683-687, 1991

The effect of formulation on the availability of sulphadiazine. Journal of Pharmacy and Pharmacology 25: Suppl:151p, 1973

Efflux of 3H-thymidine by erythrocytes from mice infected with Trypanosoma brucei brucei. Annals of Tropical Medicine and Parasitology 80(6): 581-585, 1986

Two types of nodules induced on Trifolium pratense by mutants of Rhizobium leguminosarum bv. trifolii deficient in exopolysaccharide production. Journal of Plant Physiology 147(1): 93-100, 1995

Two types of neuronal precursor cells in the mammalian retina--a short review. Journal für Hirnforschung 34(3): 335-341, 1993

Carbachol induced suppression of the n wave in guinea pig olfactory cortex slices weak antagonism by m 3 selective muscarinic antagonists hhsid and p f hhsid. British Journal of Pharmacology 101(PROC Suppl. NOV): 500P, 1990