+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The amelogenin problem a comparison of purified enamel matrix proteins

The amelogenin problem a comparison of purified enamel matrix proteins

Calcified Tissue International 27(1): 65-74

Using a combination of gel filtration and DEAE-cellulose chromatography, together with small-scale preparative polyacrylamide gel electrophoresis, 5 proteins (amelogenins) were isolated from demineralized bovine fetal dental enamel matrix. These purified proteins were characterized by amino acid analysis and gel electrophoresis. Although there are similarities in the published data between components of comparable electrophoretic mobility, there are gross differences in the reported amino acid compositions. These differences probably are due not to separative problems arising from reversible aggregations, but to inadequate comparisons of the electrophoretic and amino acid analytical data.

Accession: 006599040

Download citation: RISBibTeXText

PMID: 111790

DOI: 10.1007/bf02441163

Download PDF Full Text: The amelogenin problem a comparison of purified enamel matrix proteins

Related references

The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization. Biomaterials 27(15): 2926-2933, 2006

Interaction between the enamel matrix proteins amelogenin and ameloblastin. Biochemical and Biophysical Research Communications 323(3): 1075-1083, 2004

Production of monoclonal antibodies against enamelin and against amelogenin proteins of developing enamel matrix. Advances in Dental Research 1(2): 282-288, 1987

Molecular structure and function of matrix proteins in developing enamel--amelogenin and enamelin. Kokubyo Gakkai Zasshi. Journal of the Stomatological Society, Japan 62(3): 347-372, 1995

A developmental comparison of matrix metalloproteinase-20 and amelogenin null mouse enamel. European Journal of Oral Sciences 114 Suppl 1: 18-23; Discussion 39-41, 379, 2006

Immunochemical and immunocytochemical study of a 15 kDa non-amelogenin and related proteins in the porcine immature enamel: Proposal of a new group of enamel proteins 'sheath proteins'. Biomedical Research (Tokyo) 16(3): 131-140, 1995

Matching 4.7-Å XRD spacing in amelogenin nanoribbons and enamel matrix. Journal of Dental Research 93(9): 918-922, 2014

Dental enamel matrix: sequences of two amelogenin polypeptides. Bioscience Reports 1(10): 771-778, 1981

Dental enamel matrix sequences of 2 amelogenin poly peptides. Bioscience Reports 1(10): 771-778, 1981

Posttranslational Amelogenin Processing and Changes in Matrix Assembly during Enamel Development. Frontiers in Physiology 8: 790-790, 2017

Comparative biochemistry of the amelogenin peptides in developing dental enamel matrix. Journal of Dental Research 60(SPEC ISSUE A): 637, 1981

Antisense perturbation of amelogenin translation during enamel matrix biomineralization inhibition. Journal of Dental Research 71(SPEC ISSUE MAR): 167, 1992

Evidence for amelogenin "nanospheres" as functional components of secretory-stage enamel matrix. Journal of Structural Biology 115(1): 50-59, 1995

An amelogenin-chitosan matrix promotes assembly of an enamel-like layer with a dense interface. Acta Biomaterialia 9(7): 7289-7297, 2013

Limited proteolysis of amelogenin: toward understanding the proteolytic processes in enamel extracellular matrix. Connective Tissue Research 43(2-3): 450-455, 2002