+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

The carbohydrate moiety of human chorionic gonadotropin lack of competition with human chorionic gonadotropin for testicular receptors and anti human chorionic gonadotropin serum



The carbohydrate moiety of human chorionic gonadotropin lack of competition with human chorionic gonadotropin for testicular receptors and anti human chorionic gonadotropin serum



Hormone & Metabolic Research 11(2): 165-167



A glycopeptide fraction was prepared from human chorionic gonadotropin (HCG) by digesting the reduced, S-carboxymethylated hormone with pronase and fractionating the digest by gel exclusion chromatography. The glycopeptide fraction was estimated to contain (wt/wt) 29% sialic acid, 31% hexose, 23% hexosamine and 17% amino acids and/or peptides; the glycopeptide mixture is 83% carbohydrate compared to intact HCG which is about 30% carbohydrate. There was no cross-reactivity of the glycopeptide fraction with an antiserum directed against HCG. When corrected for minimal non-specific effects, the fraction failed to displace 125I-HCG from a rat testicular preparation even when tested at a 10,000-fold (wt/wt) excess. Any model involving carbohydrate effects in gonadotropin action must include the protein moiety as a necessary component.

(PDF emailed within 1 workday: $29.90)

Accession: 006610770

Download citation: RISBibTeXText


Related references

The carbohydrate moiety of human chorionic gonadotropin: lack of competition with HCG for testicular receptors and anti-HCG-serum. Hormone and Metabolic Research 11(2): 165-167, 1979

Fundamental and clinical evaluation of human chorionic gonadotropin radio immunoassay using iodine 125 labeled human chorionic gonadotropin and anti beta human chorionic gonadotropin system. Kaku Igaku 19(4): 607, 1982

Typical pattern of human chorionic gonadotropin human chorionic gonadotropin alpha and human chorionic gonadotropin beta serum concentrations in molar pregnancies. Acta Endocrinologica Supplementum 212: 33, 1977

The preparation of a human chorionic gonadotropin specific immunoadsorbent using human chorionic gonadotropin beta carboxyl terminal peptide antibody and its application to isolate human chorionic gonadotropin like substances from human pituitary glands. Folia Endocrinologica Japonica 60(6): 800-813, 1984

A specific radio immunoassay of a human chorionic gonadotropin beta subunit in the presence of native human chorionic gonadotropin and immunological biological and chemical characterization of human chorionic gonadotropin and its chemically modified subunits. Folia Endocrinologica Japonica 55(1): 38-53, 1979

Inhibition of human chorionic gonadotropin induced ovulation by anti human chorionic gonadotropin serum in immature mice pre treated with pregnant mare serum gonadotropin. Journal of Reproduction & Fertility 20(2): 271-277, 1969

Effects of human chorionic gonadotropin asialo human chorionic gonadotropin and human chorionic gonadotropin alpha and beta subunits upon luteinization of monkey granulosa cell cultures. Federation Proceedings: 257, 1972

The testicular response to human chorionic gonadotropin and human chorionic gonadotropin human menopausal gonadotropin treatment in patients with hypogonadotropic eunuchoidism influence of endogenous fsh. Journal of Steroid Biochemistry 20(6 PART B): 1603, 1984

Is deglycosylated human chorionic gonadotropin an antagonist to human chorionic gonadotropin? Characterization of deglycosylated human chorionic gonadotropin action in two testicular interstitial cell fractions. Biochimica et Biophysica Acta 1033(3): 226-234, 1990

Effects of photoperiod and human chorionic gonadotropin on the regulation of testicular luteinizing hormone human chorionic gonadotropin receptors in syrian hamsters mesocricetus auratus. Journal of Reproduction & Fertility 74(2): 693-700, 1985

Gonadotropin-releasing hormone effects on placental hormones during gestation: I. Alpha-human chorionic gonadotropin, human chorionic gonadotropin and human chorionic somatomammotropin. Biology of Reproduction 34(2): 245-254, 1986

Electron microscopic localization of iodine 125 labeled human chorionic gonadotropin binding to sensitive and de sensitized ovaries 7 days after pregnant mares serum gonadotropin human chorionic gonadotropin. Journal of Cell Biology 83(2 PART 2): 242A, 1979

Determination of human chorionic gonadotropin in chorio carcinoma using radio immunoassay kits for human chorionic gonadotropin and human chorionic gonadotropin beta. Acta Universitatis Palackianae Olomucensis Facultatis Medicae 101: 166-171, 1981

Estrus and pregnancy in prebubertal gilts injected with human chorionic gonadotropin:estradiol benzoate or pregnant male serum gonadotropin:human chorionic gonadotropin. Norsk veterinaertidsskrift5(7-8): 473-480, 1983

Diagnostic value of radio receptor assay for human chorionic gonadotropin and specific radio immunoassay for human chorionic gonadotropin beta subunit in subjects with ectopic human chorionic gonadotropin secretion. Acta Endocrinologica Supplementum 212: 129, 1977