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The carbohydrate moiety of human chorionic gonadotropin lack of competition with human chorionic gonadotropin for testicular receptors and anti human chorionic gonadotropin serum



The carbohydrate moiety of human chorionic gonadotropin lack of competition with human chorionic gonadotropin for testicular receptors and anti human chorionic gonadotropin serum



Hormone & Metabolic Research 11(2): 165-167



A glycopeptide fraction was prepared from human chorionic gonadotropin (HCG) by digesting the reduced, S-carboxymethylated hormone with pronase and fractionating the digest by gel exclusion chromatography. The glycopeptide fraction was estimated to contain (wt/wt) 29% sialic acid, 31% hexose, 23% hexosamine and 17% amino acids and/or peptides; the glycopeptide mixture is 83% carbohydrate compared to intact HCG which is about 30% carbohydrate. There was no cross-reactivity of the glycopeptide fraction with an antiserum directed against HCG. When corrected for minimal non-specific effects, the fraction failed to displace 125I-HCG from a rat testicular preparation even when tested at a 10,000-fold (wt/wt) excess. Any model involving carbohydrate effects in gonadotropin action must include the protein moiety as a necessary component.

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