EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The chromatin structure of specific genes part 1 evidence for higher order domains of defined dna sequence


Cell 16(4): 797-806
The chromatin structure of specific genes part 1 evidence for higher order domains of defined dna sequence
When the chromatin of Drosophila melanogaster is examined by digestion with DNAse I or micrococcal nuclease, no general structural organization above the level of the nucleosome is revealed by the cleavage pattern. The DNAse I cleavage pattern of specific regions of the Drosophila chromosome shows discrete bands with sizes ranging from a few kilobase pairs (kb) to more than 20 kb. Visualization of such higher order bands was achieved by the use of the Southern blotting technique. The DNAse I-cleaved fragments were transferred onto a nitrocellulose sheet after size fractionation by gel electrophoresis. Hybridization was then carried out with radioactively labeled cloned fragments of DNA from D. melanogaster. For the 5 different chromosomal regions examined, each gives a unique pattern of higher order bands on the autoradiogram; the patterns are different for different regions. Restriction enzyme cleavage of the fragments generated indicates that the preferential DNAse I cleavage sites in chromatin are position-specific. The chromosomal regions bounded by preferential DNAse I cleavage sites are referred to as supranucleosomal or higher order domains for purposes of discussion and analysis. The micrococcal nuclease cleavage pattern of chromatin at specific loci was also examined. In 1 case, this nuclease also cleaves at position-specific sites.


Accession: 006614591



Related references

The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence. Cell 16(4): 797-806, 1979

Higher-order chromatin domains link eQTLs with the expression of far-away genes. Nucleic Acids Research 42(1): 87-96, 2014

Roles of H1 domains in determining higher order chromatin structure and H1 location. Journal of Molecular Biology 187(4): 591-601, 1986

Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance. Plant Journal 84(1): 111-124, 2016

Intra- and inter-nucleosome interactions of the core histone tail domains in higher-order chromatin structure. Chromosoma 123(1-2): 3-13, 2014

B-globin gene family in murine erythroleukemia cells resides within two chromatin domains differing in higher order structure. Biochemistry (American Chemical Society) 23: 70-6, 1984

Beta globin gene family in murine erythroleukemia cells resides within 2 chromatin domains differing in higher order structure. Biochemistry 23(13): 2970-2976, 1984

beta-Globin gene family in murine erythroleukemia cells resides within two chromatin domains differing in higher order structure. Biochemistry 23(13): 2970-2976, 1984

Differential stability of the higher order structure of chromatin associated with genes having different transcriptional activity. Biochemical & Biophysical Research Communications 146(1): 270-276, 1987

Acetylation mimics within individual core histone tail domains indicate distinct roles in regulating the stability of higher-order chromatin structure. Molecular and Cellular Biology 28(1): 227-236, 2007