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The chromatin structure of specific genes part 2 disruption of chromatin structure during gene activity


Cell 16(4): 807-814
The chromatin structure of specific genes part 2 disruption of chromatin structure during gene activity
The chromatin structure in the active and inactive states at loci encoding the major heat shock protein in Drosophila melanogaster were compared. DNAse I and micrococcal nuclease were used as probes of higher order organization and nucleosomal integrity. Such integrity is gauged by the characteristic pattern of discrete DNA fragments produced at specific chromosomal loci by nucleolytic cleavage. The specific fragment patterns are visualized by gel electrophoresis, Southern blotting onto nitrocellulose sheets, hybridization with 32P-labeled cloned DNA containing the heat shock genes and autoradiography. A disruption in nucleosomal and possibly in higher order organization was observed, as indicated by a relative loss or smearing of the characteristic discrete DNA fragment patterns from the heat shock loci in the active state. The fragment patterns are restored when cells are allowed to recover from heat shock and these loci return to the inactive state.


Accession: 006614592



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