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The chromosomal distribution of cloned highly repetitive sequences from hexa ploid wheat triticum aestivum






Heredity 48(3): 371-376

The chromosomal distribution of cloned highly repetitive sequences from hexa ploid wheat triticum aestivum

Two highly repetitive DNA sequences from T. aestivum were isolated and purified by molecular cloning. In situ hybridization of these sequences to wheat chromosomes showed the sequence to be located predominantly at specific sites in the B genome and also chromosome 4A. Individual chromosomes could be identified by their patterns of labeling. Although 1 sequence was mostly located close to centromeres and interstititally, and the other sequence mostly at telomeres, there was a good correlation between the distribution of these sequences and the distribution of heterochromatin as revealed by C-banding. In situ hybridization of the 2 sequences to chromosomes of the related species, Secale cerale and Hordeum vulgare showed a similar correlation between the location of highly repetitive sequences and heterochromatin.


Accession: 006614639



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