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The diagnosis of bacterial ring rot of potatoes 1. pathogenicity test on eggplants


, : The diagnosis of bacterial ring rot of potatoes 1. pathogenicity test on eggplants. Phytopathologische Zeitschrift 112(3): 198-206

The eggplant test, described by Olsson (1976) and Lelliott and Sellar (1976) as a means of detecting Corynebacterium sepedonicum, the causal agent of bacterial ring rot, was tested for its reliability. Eggplants [Solanum melongena] inoculated with a pure suspension of bacterial cells developed typical chlorotic symptoms at a minimum concentration of 2 .times. 103 cells/ml inoculum. Whereas with an inoculum which consisted of potato tissue equally mixed with the bacterial suspension, the 1st symptoms developed at a concentration of 104 cells/ml. Reisolation of the pathogen by the IFAS-test or on YDC-medium was possible following inoculation at a concentration of 2 .times. 103 cells/ml. It was usually possible to isolate the bacteria in pure culture. Further solanaceous host species tested in comparison with the eggplant did not develop typical symptoms following inoculation with C. sepedonicum. On this account, the eggplant test should currently be considered as the most suitable biological assay to detect C. sepedonicum.

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