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The diagnosis of pneumococcal chest infection by countercurrent immuno electrophoresis


Journal of Applied Bacteriology 49(3): 471-478
The diagnosis of pneumococcal chest infection by countercurrent immuno electrophoresis
A simple, inexpensive method of countercurrent-immunoelectrophoresis was used to detect pneumococcal antigens in sputum, serum and pleural fluid. Antigens were detected in sputa from 69% of subjects with pneumococcal pneumonia and there was a good correlation with the certainty of the clinical diagnosis. Serum antigens were found in 12.5% of subjects with either a definite or a presumed diagnosis and in 1 case from a patient who presumably did not have pneumococcal pneumonia. The pathogenic importance of antigens in the sputa of patients with a history of chronic respiratory disease was uncertain. Countercurrent-immunoelectrophoresis was more sensitive than routine bacteriological methods of demonstraing the presence of penumococci in pleural fluids.


Accession: 006628447

PMID: 7216927



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