The diagnosis of specific hyper sensitivity to exogenous allergens by the radio allergo sorbent test 1. method and study of specificity of standard allergens in the phadebas radio allergo sorbent kit used for investigations
Bogoniowska, Z.; Wasek, Z.
Polskie Archiwum Medycyny Wewnetrznej 61(1): 11-20
In patients with bronchial asthma hypersensitivity to the following 5 groups of allergens was studied: house dust (K).sbd.3 different standard allergens, mites (R).sbd.Dermatophagoides pteronyssinus and D. farinae, epidermis of domestic animals (N).sbd.cat and dog, grass pollens (T).sbd.5 allergens from different grass species, and tree pollens (D).sbd.5 allergens from different trees. The investigations were carried out using a Phadebas-RAST [radioallergosorbent test] kit. Presence of immunoglobulin R (IgE) specific for the investigated allergens was determined in the serum. For the study, 189 patients with positive response to at least one of these allergens were chosen. On the basis of the abovementioned 5 groups of standard allergens, the patients were divided into 32 subgroups with a definite set of allergens causing hypersensitivity. The demonstrated hypersensitivity to standard house dust allergen was connected with hypersensitivity to mites and/or epidermis of domestic animals, but not with hypersensitivity to pollen allergens. Standard allergens of mites always produced cross-reactivity, but the D. pteronyssinus allergen produced a higher degree of hypersensitivity, while standard allergens of cat and dog epidermis were homogenous and could be used for differential diagnosis of hypersensitivity to these 2 epidermal allergens. Standard pollen allergens can differentiate the hypersensitivity to pollens of grass and trees, but could not differentiate between hypersensitivity to different species of grass or trees.