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The distribution of carbon 14 from uniformly distributed carbon 14 glucose in mice using whole body auto radiography



The distribution of carbon 14 from uniformly distributed carbon 14 glucose in mice using whole body auto radiography



Journal of Histochemistry and Cytochemistry 24(4): 591-600



Tissue distribution of radioactive carbon from [U-14C]glucose in the mouse in vivo was studied by whole-body autoradiography. The mice were frozen with Dry-Ice-acetone at 0.5, 1, 5 and 30 min, 1 and 24 h and 1 and 3 wk after i.p. injection of [U-14C]glucose. Whole-sagittal sections of the frozen mouse, obtained by using a microtome in a cryostat, were dried in a cryostat and autoradiographed. The resulting day autoradiographs are called untreated autoradiographs in the present work. The sections were then fixed in cold 6% (wt/vol) HClO4, dried at room temperature and again autoradiographed. Autoradiographs that have undergone this process are referred to as treated autoradiographs. In both untreated and treated autoradiographs, within 1 min following injection of the labeled glucose, the abdominal cavity had the highest autoradiographic density. At 1 h, density became highest in Harder's, sublingual and duodenal glands, large intestinal mucosa and tongue, and after 3 wks, no autoradiographic density was present.

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