The distribution of keratin type intermediate-sized filaments in so-called mixed tumour of the skin

Yoneda, K.; Kitajima, Y.; Furuta, H.; Tsuneda, Y.; Mori, S.

British Journal of Dermatology 109(4): 393-400

1983


ISSN/ISBN: 0007-0963
PMID: 6194808
DOI: 10.1111/j.1365-2133.1983.tb04613.x
Accession: 006631604

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Abstract
Epithelial cells can be distinguished from various non-epithelial cells by the presence of keratin-type intermediate-sized filaments, which can be detected by immunofluorescence microscopy, using antibodies to .alpha.-keratin. Two types of antibodies were obtained. One of them was specific for .alpha.-keratin (MW 49,000-69,000 daltons) in whole epidermis, and the other for .alpha.-keratin (MW 62,000 and 69,000 daltons) in prickle and granular cells but not in basal cells. Four cases of so-called mixed tumor of the skin were studied by immunofluorescence microscopy using these antibodies. Tumor cell nests of cuboidal and polygonal cells, tubular structures and keratinous cysts reacted with these antibodies, as did individually-dispersed tumor cells within the myxoid and chondroid matrix. All the tumor cells of the so-called mixed tumor of the skin are apparently of epithelial origin. Differences in staining intensity between these tumor cells and their specificity for these 2 antibodies are discussed with reference to keratin differentiation in tumor cells.

The distribution of keratin type intermediate-sized filaments in so-called mixed tumour of the skin