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The effect of caffeine and actinomycin d on the rate of chromosome aberrations in a culture of human lymphocytes after irradiation at various stages of the cell cycle

Sevan'kaev, A.V.; Airapetyan, L.G.

Meditsinskaya Radiologiya 22(2): 31-32

1977

Accession: 006638739
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The postradiation effect of caffeine and actinomycin D on the appearance of chromosome aberrations in a culture of human peripheral blood lymphocytes was studied after .gamma.-irradiation at various stages of the cellular cycle. Irradiation of the cell culture at doses of 200 rad was effected before the beginning of incubation (stage Go), after 20 h (stage G1), 37 h (stage S) and in a dose of 100 rad after 47 h (stage G2) from the moment of incubation. Caffeine had no effect on the appearance of chromosome aberrations in irradiation of stages Go-G1, but increased their number in stages S and G2, while the effect was most expressed in the stage of DNA synthesis. Actinomycin D manifested an effect only in stage S. The general number of chromosome aberrations in postradiation action approximately doubled the control level.

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Related References

Sevan'kaev, A.V.; Aĭrapetian, L.G. 1977: Action of caffeine and actinomycin D on the frequency of chromosome aberrations in a human lymphocyte culture after irradiation at various stages of the cell cycle Meditsinskaia Radiologiia 22(2): 31-32
Sevan'kaev, A.V.; Bogatykh, B.A.; Lychev, V.A. 1983: Effect of the time before fixation on the yield of chromosome aberrations in a culture of human lymphocytes exposed to gamma rays at different stages of the mitotic cycle Radiobiologiia 23(4): 471-475
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