The effect of di butyryl cyclic amp and glucagon on the myo cardial cell ph

Fenton, R.A.; Gonzalez, N.C.; Clancy, R.L.

Respiration Physiology 32(2): 213-224


ISSN/ISBN: 0034-5687
Accession: 006641519

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DBcAMP [dibutyl cyclic AMP) or crystalline glucagon was utilized to elevate the intracellular cAMP concentration in isolated rat hearts. Butyric acid, a metabolite of DBcAMP, was also investigated. Their effect on the intracellular pH (pHi) as determined by the distribution of [14C]DMO (Dimethadione) was investigated. Rat hearts, perfused with a recirculated modified Krebs-Henseleit solution maintained at C, were exposed to respiratory acidosis by bubbling the perfusate with 20% CO2. .alpha.- and .beta.-receptor antagonists were used to block the effects of endogenous catecholamines. Hypercapnia decreased the pHi from 7.09 to 6.82. A similar degree of hypercapnia decreased the pHi to only 6.95 in the presence of DBcAMP and to only 6.96 in the presence of glucagon. The effective buffer values .**GRAPHIC**. .DELTA. = change) were: control, 19; butyric acid, 16; DBcAMP, 139; glucagon, 148. Apparently cAMP mediates the effect of norepinephrine, which has been shown to diminish the change in pHi accompanying respiratory acidosis.