Section 7
Chapter 6,644

The effect of exogenous hemagglutinin on in vitro melanin deposition on microfilariae of brugia pahangi in hemolymph of the mosquito armigeres subalbatus

Ogura, N.

Japanese Journal of Parasitology 36(5): 291-297


Accession: 006643392

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The encapsulation and melanization of microfilariae (Mf) of Brugia pahangi in haemolymph of the mosquito, Armigeres subalbatus, were studied in vitro. Heat-killed microfilariae (hk-Mf) were heavily melanized in both haemolymph samples and cell-free haemolymph samples of the mosquitoes of which the haemagglutinin titer had been increased by injection of Bacto-Phytohemagglutinin P (PHA-P). Heavy melanization of hk-Mf also occurred in haemolymph samples of the mosquitoes when PHA-P was added after collection of haemolymph. Induction of melanin deposition by PHA-P was inhibited by N-acetyl-D-galactosamine which is a haptenic sugar against PHA-P. Many lumps of melanin deposited on live Mf in cell-free haemolymph samples prepared from the mosquitoes injectd with PHA-P as densely as on hk-Mf. Haemagglutinin seems to be one of the mediators between parasitic nematodes and the prophenoloxidase systems of host insects.

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