The effect of kinetin on the level of proteins and lipids in cucumber cotyledons cucumis sativus cultivar monastyrski
Legocka, J.; Gwozdz, E.A.; Bruska, B.
Acta Physiologiae Plantarum 7(2): 85-94
ISSN/ISBN: 0137-5881 Accession: 006647342
Kinetin markedly accelerated the degradation rate of lipids and proteins in excised maintained in darkness cucumber cotyledons (Cucumis sativus L. cv. Monastyrski). The protein decomposition was accompanied by increase of proteolytic activity, which was much higher in kinetic treated cotyledons. Analysis of protein patterns by one dimensional electrophoresis revealed six significant fractions of polypeptides. In the course of 96 hr of incubation three fractions of low mol. wt. disappeared whereas the heavier proteins remained almost at the same level. The degradation of all of the six fractions was strongly accelerated by kinetin. On the other hand kinetin after 96 hr of treatment, intensified the occurrence of polypeptides in the region of 67-70 kg. By means of two-dimensional gel electrophoresis about 450-500 polypeptides were detected. Among the proteins four groups of well defined fractions were gradually degraded with different rate and this process was accelerated by kinetin. The data suggest that cytokinins may be considered as main factors regulating the rate of reserve mobilization at the early plant developmental stages.