EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The electrogenic pump of rabbit corneal endothelium


, : The electrogenic pump of rabbit corneal endothelium. Experimental Eye Research 24(3): 249-253

De-epithelialized rabbit corneas had their stroma dissected off leaving a preparation consisting of Descemet's membrane and endothelium. At 35.degree. C, the preparation developed spontaneously a trans-endothelial potential of about 500 .mu.V, lens-side negative. The endothelial fluid pump acts by pumping an anion into the aqueous humor.

Accession: 006670392

PMID: 852523

DOI: 10.1016/0014-4835(77)90162-2

Download PDF Full Text: The electrogenic pump of rabbit corneal endothelium


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Wigham, C.G.; Turner, H.C.; Ogbuehi, K.C.C.; Hodson, S.A., 1996: Two pathways for electrogenic bicarbonate ion movement across the rabbit corneal endothelium. Amiloride (0.5 mM) inhibited the rate of entry of Na+ into corneal endothelial cells by more than half ((0.76 +- 0.10) to (0.21 +- 0.10) mu-Eq cm-2 h-1). The same concentration of amiloride caused only minimal disturbance to corneal hydration cont...

Suzuki, H., 1988: The electrogenic Na-K pump does not contribute to endothelium-dependent hyperpolarization in the rabbit ear artery. The application to smooth muscle cells of the rabbit ear artery, of either K ions after incubation with K-free solution, or of ACh produced a transient hyperpolarization of the membrane. Ouabain inhibited the former hyperpolarization but not the l...

Suzuki H., 1988: The electrogenic sodium potassium pump does not contribute to endothelium dependent hyperpolarization in the rabbit ear artery. The application of smooth muscle cells of the rabbit ear artery, of either K ions after incubation with K-free solution, or of ACh produced a transient hyperpolarization of the membrane. Quabain inhibited the former hyperpolarization but not the l...

Mayes K.R.; Hodson S., 1979: An in vivo demonstration of the bi carbonate ion pump of rabbit corneal endothelium. The bicarbonate concentration in the stromal fluid of rabbit corneas in vivo is depressed by more than 50% of its level in the aqueous humour. After allowing for ion-exchange effects, electrochemical potential effects, binding phenomena, compartme...

Geroski, D.H.; Edelhauser, H.F., 1984: Quantitation of Na/K ATPase pump sites in the rabbit corneal endothelium. In these experiments, the binding of 3H . ouabain, a specific inhibitor of Na/K ATPase, was used to quantitate the density of Na/K ATPase pump sites in the rabbit corneal endothelium. The uptake of ouabain by the corneal endothelium shows two comp...

Stiemke M.M.; Geroski D.H.; Mccartney M.D.; Cantu Crouch D.; Edelhauser H.F., 1990: The developing corneal endothelium of the rabbit changes in pump and barrier functions. Investigative Ophthalmology & Visual Science 31(4 ABSTR ISSUE): 157

Doughty, M.J.; Maurice, D., 1988: Bicarbonate sensitivity of rabbit corneal endothelium fluid pump in vitro. Stroma-endothelium preparations from rabbit corneas were mounted between two chambers and incubated with identical media on either side which contained different bicarbonate levels and, in some experiments, organic (Good's) buffers. Active fl...

Fischbarg J.; Hofer G.L.; Koatz R.A., 1980: Priming of the fluid pump by osmotic gradients across rabbit corneal endothelium. The inclusion of 5% Dextran (average MW 40,000) in solutions to preserve in vitro rabbit corneal endothelium induced a sizable osmotic flow across the preparation which was superimposed on the existing fluid transport. Even after fluid transport c...

Riley, M.V.; Miller, F.; Hodson, S.; Linz, D., 1977: Elimination of anions derived from glucose metabolism as substrates for the fluid pump of rabbit corneal endothelium. Corneal endothelial metabolism was studied to discover whether the cells might synthesize any anion from glucose at rates sufficient to account for the fluid pump. They did not. Lactate was synthesized at a rate of 0.1 .mu.Eq[microequivalent]/cm2...

Medin, W., 1992: A method for registration of toxic drug effects on corneal endothelium. Effect of gentamicin on rabbit corneal endothelium. A weight recording system (Medin & Davanger 1988, 1989), was used to demonstrate possible toxic damage of a medicament to the endothelium of rabbit corneas stored in organ culture. Seven corneas were stored in organ culture medium containing g...