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The electrophoretic and immunological characteristics of the fc positive and fc negative fractions of cba mouse splenocytes


Byulleten' Eksperimental'noi Biologii i Meditsiny 84(10): 451-454
The electrophoretic and immunological characteristics of the fc positive and fc negative fractions of cba mouse splenocytes
Conditions were established for the effective separation of Fc+ and Fc- cells, using sheep red blood cell [SRBC] monolayers, sensitized with the anti-SRBC antibodies. The method was modified by replacement of poly-L-lysine by protamine sulfate. The electrophoretic distribution profiles of Fc+ and Fc- mouse splenocytes were determined. Fc+ cells had mostly a slow electrophoretic mobility. The Fc- cells were more mobile. The Fc+ fraction contained mainly B[bone marrow-derived]-lymphocytes; the Fc- fraction was enriched with T [thymus-derived] cells. There was an extensive overlap between the electrophoretic profiles of the Fc+ and Fc- splenocytes. After the splenocyte passage through the glass bead column the overlap was much less.


Accession: 006670502



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