EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The emergence of sv 40 variants in a persistent infection of rhesus monkey kidney cells and their interaction with standard sv 40


, : The emergence of sv 40 variants in a persistent infection of rhesus monkey kidney cells and their interaction with standard sv 40. Virology 95(2): 598-614

In a persistent infection of rhesus monkey kidney cells with SV40, a heterogeneous population of variant particles emerges to replace the large plaque standard SV40 used to initiate the infection. The variants include defective interfering (DI) particles which reach detectable levels at week 6 and which are in excess of plaque forming units (PFU) by week 9. Stocks which contain an excess of DI particle activity contain an excess of particles able to produce T antigen, but not V antigen. Homotypic interference by SV40 DI particles results from a competitive interaction with standard SV40 during replication. Between 7-10 wk of persistent infection, the large-plaque standard SV40 is replaced by infectious small-plaque particles. Between 14-28 wk, the uncloned carrier culture stocks became temperature-sensitive for replication. Restriction endonuclease analysis revealed some changes in carrier culture viral DNA with the first changes evident by week 2. As late as week 57, most of the viral DNA from the carrier system yielded restriction endonuclease fragments indistinguishable from standard virus fragments.


Accession: 006670883

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Norkin, L.C., 1979: The emergence of simian virus 40 variants in a persistent infection of rhesus monkey kidney cells and their interaction with standard simian virus 40. Virology 95(2): 598-603

Norkin L.C., 1979: Small plaque variants of sv 40 from a persistent infection of rhesus monkey kidney cells. Virology 97(1): 201-206

Norkin, L.C., 1979: Small plaque variants of simian virus 40 from a persistent infection of rhesus monkey kidney cells. Virology 97(1): 201-206

Margalith M.; Margalith E.; Goldblum N., 1968: Genetic characteristics of echovirus type 9 strains selection and characterization of variants mouse human epithelial cells rhesus monkey serum monkey kidney cell monolayers baboon kidney cells. Journal of General Virology 3(1): 77-85

Kalter, S.S.; Rodriguez, A.R.; Armour, V., 1968: Production of reference enteroviruses african green monkey kidney cells baboon kidney cells rhesus monkey kidney cells human amnion cells. Applied Microbiology 16(10): 1575-1583

Norkin, L.C., 1977: Effect of input multiplicity on the establishment of sv 40 persistent infections in rhesus monkey kidney cells. Monolayer cultures of LLC-MK2 rhesus monkey kidney cells become persistently infected with SV-40 after infection at input multiplicities of 100, 10 or 1 plaque-forming unit/cell. After 3 wk, all cells of the cultures infected at a multiplicity of...

Norkin, L.C., 1977: Effect of input multiplicity on the establishment of simian virus 40 persistent infections in rhesus monkey kidney cells. Monolayer cultures of LLC-MK2 rhesus monkey kidney cells become persistently infected with simian virus 40 after infection at input multiplicities of 100, 10, or 1 plaque-forming unit per cell. After 3 weeks, all cells of the cultures infected at...

Chang, P.W.; Hsiung, G.D., 1964: Persistent infection with DA myxovirus in monkey kidney cells. FED PROC 23(2 Pt 1): 194

Asher D.M.; Gavrilov V.I.; Vyalushkina S.D.; Ratushkina L.S.; Tumyan B.G.; Zmieva R.G., 1971: Persistent infection of a continuous line of pig embryo kidney cells res with a variant of influenza ao wsn virus part 3 biological properties of influenza virus variants isolated at different periods of persistent infection. Voprosy Virusologii 16(2): 174-179

Knudson G.B.; Wozniak A.; Yan P.O.; Shiromoto R., 1991: Comparison of buffalo green monkey kidney cells with a549 mrc 5 and rhesus monkey kidney cells for virus isolation. Abstracts of the General Meeting of the American Society for Microbiology 91: 333