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The enantiomeric analysis of a mixture of all common protein amino acids by high performance liquid chromatography using a new chiral mobile phase



The enantiomeric analysis of a mixture of all common protein amino acids by high performance liquid chromatography using a new chiral mobile phase



Analytical Biochemistry 121(2): 370-377



A high-performance liquid chromatography method for the enantiomeric analysis of a mixture of all the common protein amino acids is described. The analysis is performed in 2 stages: First, the mixture is separated into groups on a cation-exchange resin, eluted and collected with volatile aqueous pyridine buffers, and the eluant is evaporated. Then the amino acids within the groups are separated into the D- and L-enantiomers on a reversed-phase column with N,N-di-n-propyl-L-alanine and cupric acetate in the aqueous mobile phase. Subnanomole sensitivity is achieved by postcolumn derivatization with o-phthaldialdehyde and subsequent fluorometric detection. Proline is detected by postcolumn derivatization with ninhydrine.

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Accession: 006670973

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PMID: 7103069



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