The esterolytic and lipolytic activities of lactobacilli detection of the esterase systems of lactobacillus casei lactobacillus plantarum lactobacillus brevis and lactobacillus fermentum

E.S.da M.; Fathallah, S.; Ezzat, N.; Desmazeaud, M.J.; Abou Donia, S.

Sciences des Aliments 6(4): 545-558

1986


ISSN/ISBN: 0240-8813
Accession: 006672890

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Abstract
The present work was undertaken with the aim of obtaining detailed information on the type and number of esterases in certain lactobacilli. As a general rule it appears that for Lactobacillus casei, L. brevis and L. fermentum strains the rate of hydrolysis increased with the increase in the number of carbon atoms in the o- or p-nitrophenyl derivative of fatty acid substrate. An opposite trend was noticed for L. plantarum. Lactobacillus casei and L. plantarum strains possess a more active esterase system when compared to L. brevis and L. fermentum. The electrophoretic study showed from the zymograms in polyacrylamide gel of L. casei a major band located at Rf = 0.48. For L. plantarum this activity was also detected. However, the enzyme showed a narrower specificity. The presence of this main esterolytic activity was noticed in all L. brevis and L. fermentum strains. The band located at Rf = 0.4 was also detected in the different strains and showed a wide specificity when compared to the same activity detected in L. casei or L. plantarum. The production of the esterase increased gradually during the growth of the organisms. The maximum specific activity was observed at the early stationary phase followed by a significant decrease in the late stationary phase. In conclusion, the difference in the specificity of the enzymes is suggested as new criteria for the differentiation of some closely related lactobacilli like L. plantarum and L. casei.