EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The extraction and diagnosis of corynebacterium sepedonicum from diseased potato tubers



The extraction and diagnosis of corynebacterium sepedonicum from diseased potato tubers



Bulletin OEPP 14(2): 147-152



C. sepedonicum causes a severe disease in potato and it is therefore necessary for the method of its diagnosis to be specific and for the extraction technique to ensure that even small amounts of bacteria will be found. By using indirect immunofluorescence staining it was possible to get a reliable diagnosis. For this purpose a specific serum was produced. To find the best technique for extracting the bacteria from potato tubers, 2 methods were compared. One was based on maceration of the plant material in a Waring blender followed by centrifugation. Immunofluorescence staining was done on the resuspended sediment. The other method was carried out by cutting the potatoes into small pieces (about 1 cm3) and transferring them to a bottle containing sterile water. The bottle was shaken for 3 or 16 h, and samples for immunofluorescence tests were taken from the fluid. Centrifugation could be used if necessary before immunofluorescence staining. The most reliable technique for extraction was to shake the plant material for 16 h followed by centrifugation. If there are heavy symptoms, dilution of the extracted material may be necessary.

(PDF emailed within 0-6 h: $19.90)

Accession: 006675242

Download citation: RISBibTeXText

DOI: 10.1111/j.1365-2338.1984.tb01862.x



Related references

Investigations on the spread of ring rot of potato (Corynebacterium sepedonicum (Spieck. & Kotth.) Skapt. & Burkh.) from seed tubers to plants and daughter tubers and from diseased plants to healthy ones in the field. Roczniki Nauk Rolniczych, E 1978; 7(2): 37-48, 1977

Scheme for the detection and diagnosis of the ring rot bacterium Corynebacterium sepedonicum in batches of potato tubers. 1987

The indexing of potato tubers for bacterial ring rot, Corynebacterium sepedonicum. Canadian Jour Microbiol 8(1): 65-70, 1962

Comparison of 3 identification methods for corynebacterium sepedonicum in potato tubers. Potato Research 22(2): 133-148, 1979

Comparison of three identification methods for Corynebacterium sepedonicum in potato tubers. Potato Research 22(2): 133-147, 1979

Cross reactions of corynebacterium sepedonicum anti sera with soil bacteria associated with potato tubers. Netherlands Journal of Plant Pathology 90(1): 23-28, 1984

Latent infections Corynebacterium sepedonicum in potato seed tubers imported from Canada in the period 1978-79. Informatore fitopatologico 31(5): 3-9, 1981

The indexing of potato tubers with a view toward the detection of bacterial ring rot (Corynebacterium sepedonicum)(Spieck and Kott.) Skaptason and Burkholder. Canadian Journal of Microbiology 8: 65-70, 1962

Rapid detection of Clavibacter michiganensis ssp. sepedonicus syn. Corynebacterium michiganense spp. sepedonicum (Cms) in the tissue of potato tubers and aubergine plants by gas chromatography of whole cell fatty acids. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 42(6): 87-90, 1990

Investigation of different strains of the ring rot pathogen (Corynebacterium sepedonicum) and blackleg pathogen (Pectobacterium phytophthorum) isolated from potato tubers. Mezhdunarodnyi Sel' skokhozyaistvennyi Zhurnal ( 4): 54-55, 2000

Studies on Corynebacterium sepedonicum on potato. I. Observations on the incidence of C. sepedonicum Spiek. Dows. in the Tai-Koan-Ryeng zone and experiments on its control. Nong-sa Si-hem Yen-ku Po-ko/Res. Rep. Office Rur. Dev, Suwon, 11: 2, 1-15, 1968

Bacterial ring rot of potato (Corynebacterium sepedonicum Spiek. et Kotth. Skapt. et Burkh.), with special reference to the diagnosis of the pathogen. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 35(10): 155-156, 1983

Relation between the distribution of bacteria and wilt in plants parasitized by Corynebacterium sepedonicum (Spieckermann & Kotthoff) Skaptason & Burkholder. Multiplication and migration of Corynebacterium sepedonicum, in the tissues of infected plants. Preliminary notes. Bol. Soc. broteriana. 20: 5-31, 1946

Nutrition of corynebacterium sepedonicum abstract potato d. Recherches Agronomiques (11): 24-25, 1965, 1966

Bioassay of Corynebacterium sepedonicum using potato plantlets. American Potato Journal 65(8): 501, 1988