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The extraction and diagnosis of corynebacterium sepedonicum from diseased potato tubers

The extraction and diagnosis of corynebacterium sepedonicum from diseased potato tubers

Bulletin OEPP 14(2): 147-152

C. sepedonicum causes a severe disease in potato and it is therefore necessary for the method of its diagnosis to be specific and for the extraction technique to ensure that even small amounts of bacteria will be found. By using indirect immunofluorescence staining it was possible to get a reliable diagnosis. For this purpose a specific serum was produced. To find the best technique for extracting the bacteria from potato tubers, 2 methods were compared. One was based on maceration of the plant material in a Waring blender followed by centrifugation. Immunofluorescence staining was done on the resuspended sediment. The other method was carried out by cutting the potatoes into small pieces (about 1 cm3) and transferring them to a bottle containing sterile water. The bottle was shaken for 3 or 16 h, and samples for immunofluorescence tests were taken from the fluid. Centrifugation could be used if necessary before immunofluorescence staining. The most reliable technique for extraction was to shake the plant material for 16 h followed by centrifugation. If there are heavy symptoms, dilution of the extracted material may be necessary.

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Accession: 006675242

Download citation: RISBibTeXText

DOI: 10.1111/j.1365-2338.1984.tb01862.x

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