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The first international standard for antibodies to double stranded DNA

Annals of the Rheumatic Diseases 47(9): 740-746

The first international standard for antibodies to double stranded DNA

This paper announces the availability of the first international standard for anti-double-stranded DNA (anti-dsDNA). The material, coded Wo/80, was obtained after recalcification of plasma taken from a patient with systemic lupus erythematosus. Vials were filled with 500 microliters serum and freeze dried. The serum contains no other autoantibodies in measurable quantities. The vials should be stored at -20 degrees C. The standard should be used for establishing national, regional, or local standards. In eight laboratories satisfactory results with the immunofluorescence technique on Crithidia luciliae were obtained; the titres varied between 1/20 and 1/640 (mean 1/160). In seven laboratories the Farr assay, with the so called 'Amersham kit', was performed. At a dilution of 1:40 a mean binding percentage of about 50% was observed. After reconstitution with 500 microliters of distilled water, the vial contains 100 IU/500 microliters or 200 IU/ml. The standard can be obtained from the custodian of WHO: Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, PO Box 9190 1006 AD Amsterdam, The Netherlands.

Accession: 006677951

PMID: 3052322

DOI: 10.1136/ard.47.9.740

Related references

Lange A.; Jacak A.; Garncarek D., 1978: Diagnostic specificity of auto antibodies part 4 a double antibody solid phase radio immunoassay for dna antibodies significance of double stranded dna and single stranded dna antibodies with test standardization attempts. Archivum Immunologiae et Therapiae Experimentalis 26(1-6): 893-897

Taylor R.P.; Horgan C., 1984: Quantitative determination of anti double stranded dna antibodies and antibody double stranded dna stoichiometries in prepared soluble complement fixing antibody double stranded dna immune complexes. The complement-mediated binding of prepared, soluble 125I-7S IgG antibody 3H-ds [double stranded] DNA immune complexes to human red blood cells (RBC) was investigated quantitatively. These studies were performed by using a detailed modification of...

Suenaga R.; Abdou N.I., 1991: Expression of inactive stage anti double stranded dna private idiotypes on anti single stranded dna antibodies and not on anti double stranded dna antibodies in a lupus patient during active cns stage of the disease. Arthritis & Rheumatism 34(9 SUPPL): S99

Sontheimer, R.D.; Gilliam, J.N., 1978: An immunofluorescence assay for double-stranded DNA antibodies using the Crithidia luciliae kinetoplast as a double-stranded DNA substrate. Circulating antibodies to dsDNA are found predominately in SLE and are seen most often in those patients with active systemic disease, particularly severe lupus glomerulonephritis. An IIF technique for measuring these antibodies has recently been...

Saito, M.; Uechi, Y.; Nakabayashi, K.; Nagasawa, T., 1994: Measurement of antibodies to double-stranded and single-stranded DNA using bacteriophage lambda DNA as the antigen--avidity of antibodies to DNA in patients with lupus nephritis. IgG-class antibodies to double-stranded (ds) and single-stranded (ss) DNA in patients with collagen disease were measured by enzyme-linked immunosorbent assay (ELISA) using bacteriophage lambda DNA as the antigen. Moreover, avidity of IgG-class an...

Miyawaki, S.; Mineshige, H.; Kawamura, M., 1992: Reassessment of measurement of anti double-stranded DNA antibodies by Farr's assay using double-stranded DNA from E. coli and application of DNA binding Activities in high salt solution. Farr's assay using double-stranded (ds) DNA from E. coli is a most sensitive and specific method for the detection of anti ds-DNA antibodies in patients with systemic lupus erythematosus (SLE). Because of the lack of sufficient DNA antigens,...

Taylor R.P.; Kujala G.; Edberg J.C.; Foreman P.; Davis J.S.Iv; Wright E., 1986: The kinetics of clearance of human antibody double stranded dna immune complexes from rabbit circulation effects of double stranded dna size rheumatoid factor and reduction and alkylation of the antibodies. We have conducted in vivo investigations of the rate of clearance of prepared antibody/dsDNA immune complexes from the circulation of rabbits and have correlated the results with some in vitro immunologic properties of the complexes. Those complex...

Compans, RW.; Bishop, DHL., 1983: Double-stranded RNA viruses. Proceedings of the First International Symposium on Double-Stranded RNA Viruses, held October 5-10, 1982, at Frenchman's Reef, St. Thomas, U.S. Virgin Islands. The many papers published in this report on the First International Symposium on Double-Stranded RNA Viruses, held in the Virgin Islands in October 1982, are arranged under sections on RNA structure, biosynthesis and coding; proteins and translati...

Tsuchiya, K.; Kiyosawa, K.; Imai, H.; Sodeyama, T.; Furuta, S., 1994: Detection of anti-double and anti-single stranded DNA antibodies in chronic liver disease: significance of anti-double stranded DNA antibody in autoimmune hepatitis. Anti-DNA antibodies were determined by an enzyme-linked immunosorbent assay in 116 patients with chronic liver disease consisting of 21 cases of autoimmune hepatitis (AIH), 17 of primary biliary cirrhosis (PBC), and 78 of non-autoimmune-type of ch...

Thoburn R.; Koffler D.; Kunkel H.G., 1971: Distribution of antibodies to native dna single stranded dna and double stranded rna in mouse sera. Proceedings of the Society for Experimental Biology & Medicine 136(3): 711-714