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The formation of a root specific iso peroxidase as an indicator of root primordium differentiation in pith of tobacco nicotiana tabacum cultivar samsun



The formation of a root specific iso peroxidase as an indicator of root primordium differentiation in pith of tobacco nicotiana tabacum cultivar samsun



Science Reports of Faculty of Agriculture Kobe University 14(1): 85-92



The isoperoxidases in excised pith of tobacco plant (N. tabacum cv. 'Samsun') was examined by starch-gel electrophoresis. In cultured pith pieces, 4 cathodic isoperoxidases designated Cx, Cy, C4 and C5 developed within 2 days after inoculation. In 6 cm long stem section taken from the internode at 12-24 cm of distance from apex, a root-specific isoperoxidase C5 was induced only in the original basal pole, while the 3 others were induced in both apical and basal poles. The difference may demonstrate C5 to be induced in response not to injury but to the change which would reflect a certain physiological change leading to differentiation of root primodium. Actinomycin D did not inhibit the development of injury-dependent isoperoxidases but completely inhibited the development of C5. In the presence of 2, 4-D in the medium, C5 was never developed in excised pith without significant diminition of ininjury-dependent isoperoxidases.

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Accession: 006679222

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