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The freeing of potato clones from viruses by meristem tip culture 1. some factors affecting plantlet regeneration from meristem tip cultures and virus eradication in plants regenerated






Science Reports of Faculty of Agriculture Kobe University 16(1): 43-54

The freeing of potato clones from viruses by meristem tip culture 1. some factors affecting plantlet regeneration from meristem tip cultures and virus eradication in plants regenerated

Studies were carried out on some factors affecting plantlet regeneration from meristem-tip cultures and also on the virus assay for the meristem-derived plants, using [Solanum] andigena potato clone (2n = 48) and its diploid relative S. rybinii (2n = 24). Plantlets regenerated with the frequencies of 13.7, 30.0 and 78.3% in the meristem-tips excised from adult plants, sprouts emerging naturally from tubers and sprouts from tubers in cold storage, respectively. The larger meristem-tips, about 0.5 mm tall, with 2 layers of leaf primordia, showed about 17% higher frequency of plantlet regeneration than in the smaller ones, about 0.3 mm tall, with 1 layer of leaf primordia. The mean frequencies of plantlet regeneration as affected by growth substances were 25.4, 14.2, 47.6 and 36.3% in no, BA [benzyl adenine] 10 ppm, IAA 1 ppm and BA 1 ppm + IAA 1 ppm + GA3 10 ppm supplements, respectively. S. rybinii, despite being a heavily virus-infected clone, regenerated plantlets more frequently from the meristem-tips than in moderately virus-infected andigena clone, their frequencies being 37.5% in the former and 24.4% in the latter. The results of virus assay indicated that while all of plants derived from larger meristem-tips were virus-free, 90.0% of those from smaller ones were so. Taking the above plantlet regeneration frequencies, the production rate of virus-free plants is assessed to be 39.5% in the former and 26.1% in the latter. Other factors such as plant ages and growth supplements did not appear to affect virus eradication in meristem-derived plants.

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Accession: 006679915



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