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The frequency of marker changes in sugarcane saccharum plants regenerated from callus culture

, : The frequency of marker changes in sugarcane saccharum plants regenerated from callus culture. Plant Cell Tissue & Organ Culture 3(3): 201-210

Leaf tissue from 5 sugarcane clones with distinctive markers was cultured on a medium favoring callus growth. Transferred to a differentiation medium, calli produced > 5000 plants. Plants differentiated from 2 clones with stem markers exhibited a high rate of remission of the marker, but the marker reappeared in the vegetative progeny of these plants, and remission was, therefore, transient. Plants differentiated from callus from 2 clones with leaf markers showed a low rate of remission (2 or 3/1000) of the marker and the vegetative progeny was stable. A clone with variegated leaves produced plants with the majority having green leaves, some albino, and some variegated, suggesting that plant differentiation may start with > 1 cell. Permanent phenotypic change may result from tissue culture, but such changes are not frequent and may be confounded by temporary alterations by chimeras formed in the process of differentiation.

Accession: 006680099

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Related references

Irvine, J.E., 1984: The frequency of marker changes in sugarcane plants regenerated from callus culture. Leaf tissue from 5 clones with distinctive markers (leaf freckle, leaf blotch, zigzag stalk, missing internode and leaf variegation) was cultured on a medium favouring callus growth. Transferred to a differentiation medium, calluses produced over...

Irvine, J.E.; Benda, G.T.A.; Legendre, B.L.; Machado, G.R.. Jr.;, 1991: The frequency of marker changes in sugarcane plants regenerated from callus culture ii. evidence for vegetative and genetic transmission epigenetic effects and chimeral disruption. This study investigates the frequency of apparent and permanent expression of marker change following two types of tissue culture, conventional callus and direct regeneration cultures, and for two markers it relates this frequency to that followin...

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