EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The function of ia positive dendritic cells and ia negative dendritic cell precursors in thymocyte mitogenesis to lectin and lectin plus interleukin 1


, : The function of ia positive dendritic cells and ia negative dendritic cell precursors in thymocyte mitogenesis to lectin and lectin plus interleukin 1. Journal of Experimental Medicine 167(1): 149-162

The response of thymocytes to lectin is a standard tissue culture model for identifying cytokines such as IL-1 that are required for thymocyte mitogenesis. To study accessory cell requirements for these responses, it was necessary to deplete endogenous accessory cells with two techniques: anti-Ia and complement, and passage over nylon wool. Proliferation to Con A was then restored with 0.1-0.3% exogenous splenic dendritic cells, or 30-fold higher levels of peritoneal macrophages. The "costimulatory" action of IL-1, whereby responses to lectin were enhanced 3-10-fold, required the presence of dendritic cells. This effect of IL-1 could be reproduced by culturing the dendritic cells for 12 h in 1 U/ml human or murine rIL-1.alpha. before addition to the thymocyte proliferation assay. The function of IL-1-treated dendritic cells was not blocked by a neutralizing anti-IL-1 antibody. The endogenous population of thymic accessory cells was partially characterized. A trace (0.1-0.3%) fraction of Ia+, Ig-, plastic nonadherent dendritic cells was visualized and enriched to a level of 1-10% by depleting CD4+, CD8+, and Ig+ lymphocytes. When this double-negative population was cultured with IL-1 and washed, the treated thymic dendritic cells were 10-fold more active as accessory cells. When the CD4-, CD8-, Ig- populations were depleted dendritic cells with anti-Ia and complement, the subsequent addition of IL-1 had a second effect. Ia+ dendritic cells redeveloped over a 2-d interval, and they exhibited the same properties as resident dendritic cells in thymus and spleen. The majority were lysed by 33D1 anti-dendritic cell mAb and complement, lacked Fc receptors, and acted as powerful stimulators of the MLR and Con A mitogenesis. The development of dendritic cells did not occur with IL-2, -3, -4 or granulocyte/macrophage colony-stimulating factor or in nylon-nonadherent populations. The IL-1-dependent, Ia- precursor was not detectable in bone marrow. These results begin to analyze the endogenous accessory function of the thymus in culture. Dendritic cells actively stimulate thymocyte mitogenesis. The mitogenic action of IL-1 involves effects on resident Ia+ dendritic cells as well as a new population of thymic, Ia- precursors.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006680382

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Anonymous, 2012: Maternal Super-obesity and Perinatal Outcomes. To determine the effect of maternal super-obesity (BMI ?5 kg/m<sup loc="post">2</sup>) compared to morbid obesity (BMI 4-49.9 kg/m<sup loc="post">2</sup>) or obesity (BMI 3-39.9 kg/m<sup loc="pos...

Tirelli, Ezio; Witkin, Jeffrey M., 1995: Differential Effects of Direct and Indirect Dopamine Agonists on the Induction of Gnawing in C57Bl/6J Mice. The ability of indirect dopamine agonists to induce gnawing in male C57Bl/6J mice was compared to that of direct dopamine agonists acting at dopamine D-1 or D-2 receptor subtypes. Holes left by the mice on the corrugations of packing cardboard wer...

Jordan, Jp, 1988: From shoats to geeps: what hath research wrought?. Journal of animal science 66(8): 2117-2120

Mikhailov, S. S., 1963: Reflex responses of blood pressure and breathing upon stimulation of the cavernous venous sinus. The cavernous venous sinus (CVS) of dogs were subjected to various stimuli and drugs; the ensuing changes in arterial blood pressure (ABP) and venous blood pressure (VBP), and the changes in respiratory movements (RM) were recorded kymographically...

Suknev V. S.; Kitsul V. I.; Lazebnik Yu D.; Brovkin A. A., 1971: Quantitative determination of CO (sub 2) in cordierites by infrared spectroscopy and chemical analysis. Transactions (Doklady) of the US

Whitfield, F. B.; Stanley, G., 1977: The structure and stereochemistry of edulan i and edulan ii and the stereochemistry of the 2 5 5 8a tetra methyl 3 4 4a 5 6 7 8 8a octa hydro 2h 1 benzopyrans. Edulan I and II, 2 important trace components of the juice of passionfruit Passiflora edulis (Sims), were shown by micro-reaction GLC, by micro-spectrometry (ms [mass spectrometry], NMR, IR and UV) and by comparison with synthetic products to be t...

Wachholder, K.; Beckmann, A.; Walter, H., 1949: Changes in the white blood cells after absorption of food. Alimentary leucopenia and alimentary leucocytosis. Blood was taken from the ear, leukocytes were counted in a Thoma-Zeiss chamber, differential counts were made by Schilling's method, basal metabolism and S.D.A. were estimated by the Knipping apparatus, blood sugar was estimated by the Hagedo...

Knorre A.G., 1967: Evolutionary histology and the paths of its development in the ussr inst electron microscopy. Arkhiv Anatomii Gistologii i Embriologii 52(9): 22-38

Chait, Alan; Han, Chang-Yeop; Oram, John-F; Heinecke, Jay, W., 2005: Thematic review series: The Immune System and Atherogenesis. Lipoprotein-associated inflammatory proteins: markers or mediators of cardiovascular disease?. In humans, a chronically increased circulating level of C-reactive protein (CRP), a positive acute-phase reactant, is an independent risk factor for cardiovascular disease. This observation has led to considerable interest in the role of inflammat...

Andrianjakavah, R. P.; Salvi, S.; Beziat, D.; Guillaume, D.; Rakotondrazafy, M.; Giuliani, G., 2009: Nature of mineralizing fluids and P-T conditions of the Ianapera pegmatite-related emerald deposit. Proceedings of the European Current Research on Fluid Inclusions (ECROFI) 20: 5-6