+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The function of teichoic acids in cation control in bacterial membranes

The function of teichoic acids in cation control in bacterial membranes

Biochemical Journal 132(1): 83-93

1. The effects of teichoic acids on the Mg(2+)-requirement of some membrane-bound enzymes in cell preparations from Bacillus licheniformis A.T.C.C. 9945 were examined. 2. The biosynthesis of the wall polymers poly(glycerol phosphate glucose) and poly(glycerol phosphate) by membrane-bound enzymes is strongly dependent on Mg(2+), showing maximum activity at 10-15mm-Mg(2+). 3. When the membrane is in close contact with the cell wall and membrane teichoic acid, the enzyme systems are insensitive to added Mg(2+). The membrane appears to interact preferentially with the constant concentration of Mg(2+) that is bound to the phosphate groups of teichoic acid in the wall and on the membrane. When the wall is removed by the action of lysozyme the enzymes again become dependent on an external supply of Mg(2+). 4. A membrane preparation that retained its membrane teichoic acid was still dependent on Mg(2+) in solution, but the dependence was damped so that the enzymes exhibited near-maximal activity over a much greater range of concentrations of added Mg(2+); this preparation contained Mg(2+) bound to the membrane teichoic acid. The behaviour of this preparation could be reproduced by binding membrane teichoic acid to membranes in the presence of Mg(2+). Addition of membrane teichoic acid to reaction mixtures also had a damping effect on the Mg(2+) requirement of the enzymes, since the added polymer interacted rapidly with the membrane. 5. Other phosphate polymers behaved in a qualitatively similar way to membrane teichoic acid on addition to reaction mixtures. 6. It is concluded that in whole cells the ordered array of anionic wall and membrane teichoic acids provides a constant reservoir of bound bivalent cations with which the membrane preferentially interacts. The membrane teichoic acid is the component of the system which mediates the interaction of bound cations with the membrane. The anionic polymers in the wall scavenge cations from the medium and maintain a constant environment for the membrane teichoic acid. Thus a function of wall and membrane teichoic acids is to maintain the correct ionic environment for cation-dependent membrane systems.

(PDF same-day service: $19.90)

Accession: 006680446

Download citation: RISBibTeXText

PMID: 4722902

DOI: 10.1042/bj1320083

Related references

Function of teichoic acids and effect of novobiocin on control of Mg2+ at the bacterial membrane. Nature: New Biology 229(2): 53-55, 1971

Bacterial cell walls and membranes. Discovery of the teichoic acids. Bioessays 10(6): 207-210, 1989

Teichoic acids in cell walls and membranes of bacteria. Essays in Biochemistry 8: 35-77, 1972

Bacterial antigens teichoic acids. Methods in Immunology & Immunochemistry : 40-44, 1967

Teichoic acids from bacterial walls. Nature 184(4682): 247-248, 1959

Teichoic acids in bacterial coaggregation. Microbiology 146: 1257-1258, 2000

Teichoic Acids And The Bacterial Cell Wall. Endeavour 23: 33-37, 1964

Biological properties of bacterial teichoic acids. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii: 12-18, 1982

Polyelectrolyte nature of bacterial teichoic acids. Journal of Bacteriology 118(2): 606-615, 1974

The interaction of concanavalin A with teichoic acids and bacterial walls. Biochemical Journal 123(4): 665-667, 1971

Composition of teichoic acids from a number of bacterial walls. Nature 184: 247-248, 1959

Soluble macromolecular complexes involving bacterial teichoic acids. Journal of Bacteriology 124(1): 341-347, 1975

Characteristics of the structure, biosynthesis and biological functions of bacterial teichoic acids. Uspekhi Sovremennoi Biologii 75(3): 357-378, 1973

Teichoic acids in the walls of staphylococci I Serological investigations on teichoic acids from the walls of staphylo-cocci. 1964

Direction of chain extension during the biosynthesis of teichoic acids in bacterial cell walls. Nature 221(5181): 665-666, 1969