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The function of the histidine transfer rna iso accepting species in hemo globin synthesis

, : The function of the histidine transfer rna iso accepting species in hemo globin synthesis. Journal of Biological Chemistry 253(17): 5964-5970

Rabbit reticulocytes contained 2 RNA isoaccepting species for histidine as resolved by various chromatographic methods, while rabbit liver contains only one. These isoacceptors cannot be distinguished by coding properties, consistent with the 'Wobble Hypothesis'. Their function in Hb synthesis in reticulocyte lysates was investigated. Each of the tRNA isoacceptors of reticulocytes and the tRNA species of liver can incorporate histidine into positions in Hb encoded by both of the histidine code words, CAC and CAU. Each can incorporate histidine into all of the histidine-containing positions of Hb. Even in experiments where probably the 2 histidine tRNA species of reticulocytes are placed together in a lysate and are in competition with each other, each incorporates histidine into all of the histidine-containing positions. There is no evidence by either of the tRNA species. The 2 species are attached to reticulocyte ribosomes in the same proportion as they occur in the reticulocyte, also suggesting that neither of them is used preferentially in Hb synthesis. The 1st of the 2 reticulocyte histidine isoacceptors and the histidine tRNA of rabbit liver contain Q base.

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