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The functional relationship between the polymerization and catalytic activity of beef liver glutamate dehydrogenase part 3 analysis of thusius critique


, : The functional relationship between the polymerization and catalytic activity of beef liver glutamate dehydrogenase part 3 analysis of thusius critique. Journal of Molecular Biology 129(1): 37-44

Thusius' critique (1977) of the authors' work on the functional relationship between the equilibrium polymerization and catalytic activity of beef liver glutamate dehydrogenase is refuted on a point by point basis. Thusius' critique is primarly based on data relating to the kinetics of the polymerization-depolymerization reaction. Such data are not in contradiction to the equilibrium thermodynamic analysis, for this analysis makes absolutely no predictions regarding the kinetics of the polymerization-depolymerization reaction. The important functional relationship between the polymerization and allosteric control of beef liver glutamate dehydrogenase has been clearly demonstrated on a purely experimental basis. The theoretical model is the only proposed model capable of quantitatively explaining the available data on the detailed equilibrium polymer distribution and associated level of catalytic activity as functions of effector and enzyme concentrations.

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Related references

Cohen, R.J.; Benedek, G.B., 1979: The functional relationship between the polymerization and catalytic activity of beef liver glutamate dehydrogenase. III. Analysis of Thusius' critique. Thusius' critique (1977) of our work (Cohen et al., 1975,1976; Cohen & Benedek, 1976), on the functional relationship between the equilibrium polymerization and catalytic activity of beef liver glutamate dehydrogenase, is refuted on a poi...

Cohen, R.J.; Jedziniak, J.A.; Benedek, G.B., 1976: The functional relationship between polymerization and catalytic activity of beef liver glutamate dehydrogenase part 2 experiment. Quasi-elastic light-scattering spectroscopic measurements of the polymer distribution and parallel determinations of the catalytic activity of bovine glutamate dehydrogenase in solution are presented in order to examine the relationship between th...

Cohen, R.J.; Benedek, G.B., 1976: The functional relationship between polymerization and catalytic activity of beef liver glutamate dehydrogenase part 1 theory. The reversible polymerization of beef liver glutamate dehydrogenase may play an important role in the allosteric control of this enzyme. A simple quantitative model for analyzing the detailed relationship between the distribution of enzyme polymer...

Thusius, D., 1977: Does a functional relationship exist between the polymerization and catalytic activity of beef liver glutamate dehydrogenase. The recent work of Cohen and Benedek and Cohen and associates on the apparent interdependence of beef liver glutamate dehydrogenase catalytic activity and degree of polymerization was examined in light of previously published equilibrium and kinet...

Cohen, R.J.; Benedek, G.B., 1976: The functional relationship between polymerization and catalytic activity of beef liver glutamate dehydrogenase. I. Theory. Journal of Molecular Biology 108(1): 151-178

Cohen, R.J.; Jedziniak, J.A.; Benedek, G.B., 1976: The functional relationship between polymerization and catalytic activity of beef liver glutamate dehydrogenase. II. Experiment. Journal of Molecular Biology 108(1): 179-199

Zeiri, L.; Reisler, E., 1978: Uncoupling of the catalytic activity and the polymerization of beef liver glutamate dehydrogenase. The recent work of Cohen and Benedek and Thusius has revived the issue of the functional relationship between the polymerization and the catalytic activity of beef liver glutamate dehydrogenase. Experimental evidence shows that the polymerization...

Kempfle M.; Mosebach K O.; Blanke C., 1970: The influence of effectors on the molecular weight and activity of glutamate dehydrogenase from beef liver in vitro part 2 the activity of glutamate dehydrogenase and its subunits. Zeitschrift fuer Naturforschung Teil B Anorganische Chemie Organische Chemie Biochemie Biophysik Biologie 25(3): 273-276

Eisenkraft B.; Veeger C., 1968: Effect of 2 oxo glutarate on the catalytic activity and stability of enz glutamate dehydrogenase beef liver. Biochimica et Biophysica Acta 167(2): 227-238

Iakovleva, V.I.; Gubnitskiĭ, L.S., 1973: Thermostability of the catalytic activity and allosteric properties of crystalline L-glutamate dehydrogenase from beef liver. Biokhimiia 38(6): 1163-1168