The growth of tritiated thymidine labeled lactobacillus casei immobilized in poly acrylamide gel lattice

Divies, C.

Annales de Microbiologie (Paris) 128B(3): 349-358


Accession: 006685489

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When 3H6-thymidine labeled bacteria (1.2 disintegration/cell per day) are immobilized in a polyacrylamide gel lattice, the decimal reduction time for their malic acid decarboxylase activity is extended to 38 days. Thus, the growth of bacteria in the gel lattice is very poor. The DNA turnover rate during the exponential growth phase for the free cells and the DNA specific activity of the population of immobilized cells were examined. The viability of bacteria isolated from the gel lattice decreases with time, as does their thermal stability for malic acid decarboxylase activity. This activity is partially restored after 65 days. With respect to the protein, deoxyribose and ribose constituents, the protein/deoxyribose, protein/ribose and deoxyribose/ribose ratios fluctuats considerably with time. These fluctuations are more marked than those observed for the free cells during their different growth phases.