The growth of tumour xenografts in thymectomized high dose irradiated mice reconstituted with syngeneic bone marrow cells incubated with anti-thymocyte serum

Franks, C.R.; Bishop, D.; Reeson, D.

British Journal of Cancer 33(1): 112-115


ISSN/ISBN: 0007-0920
PMID: 766805
Accession: 006685491

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The combination of irradiation and antithymocyte serum (ATS) incubated bone marrow cells enhanced subcutaneous growth of tumor xenografts derived from HeLa human cervical cancer cells and solid tumor implants. The 5% thymus-derived (T) cell population introduced with the bone marrow cell inoculum may be the signficant factor controlling the inhibition of implanted tumor growth. It is not clear why the HeLa tumors regressed in the 900 rad plus ATS mice at day plus 63. The combination of a return to immunological competence and the relatively small tumor load may be responsible for the gradual regression observed. This did not occur in the bladder tumor at 900 rads plus ATS because by day plus 63 the surface area of the tumors was 4 times as great as in the mice with the HeLa tumors, indicating a well established graft. Growth of both tumors was slow in the 900 rad mice. This is probably due to the presence of the small pool of immunologically competent cells introduced with the bone marrow cell inoculum, against which the establishment of tumor growth is achieved. The comparatively slow growth rates initially obtained at 1200 rads, with or without ATS, may be due to subcutaneous damage following irradiation, thus inhibiting nutrition of the implanted tumors. The combination of 900 rads plus bone marrow cells incubated with ATS enhanced growth of implanted tumors and, in the case of solid tumors, sustained enhanced growth in excess of 22% at day plus 70 when compared with 900 rad mice.