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The hemagglutination inhibition hai test in the diagnosis of bovine coronavirus infections



The hemagglutination inhibition hai test in the diagnosis of bovine coronavirus infections



Revue Roumaine de Medecine Virologie 37(4): 253-256



The main aim of the work was to obtain a viral corpuscle suspension with high hemagglutinating activity to be used as an antigen in HAI test. The bovine coronavirus SC-1 strain was cultivated on ovine foetal renal cells and gave a suspension with a hemagglutinating titer of 1 :64.sbd. 1 :128. A monospecific anti-coronavirus immune serum to be used in the HAI test, too, was prepared by hyperimmunization of a gnotobiotic calf. Its hemagglutinating and neutralizing titers were of 1:2560 and, respectively 8.5 log. The HAI test performed using the following reagents: the coronavirus suspension as a hemagglutinating antigen, a 1% suspension of mouse red blood corpuscles, the monospecific anti-coronavirus immune serum as a positive control, and a negative serum. 265 serum samples were investigated, collected from cows and calves in 18 farms where neonatal gastroenteritis was diagnosed. In 72.4% out of these samples there were positive results in the HIA test.

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