+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers

Biochemical Journal 186(1): 235-242
The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers
About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labeled folate was incorporated into 2 folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, although less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0 nmol/g) and 10-formylpteroyltetraglutamate (0.25 nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase [glutamate carboxypeptidase, EC] treatment).

(PDF 0-2 workdays service: $29.90)

Accession: 006689561

Related references

The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers. Biochemical Journal 186(1): 235-242, 1980

Synthesis and in vitro/in vivo evaluation of 99mTc-labeled folate conjugates for folate receptor imaging. Nuclear Medicine and Biology 38(4): 557-565, 2011

Synthesis and evaluation of folate-bis and folate-CYCLAM conjugates for possible use as folate-receptor-targeted copper radiopharmaceuticals. Journal of Labelled Compounds & Radiopharmaceuticals 42(SUPPL 1): S821-S823, June, 1999

Impact of high and low folate diets on tissue folate receptor levels and antitumor responses toward folate-drug conjugates. Journal of Pharmacology and Experimental Therapeutics 327(3): 918-925, 2008

Liver folate conjugates related to nutritional folate sufficiency and a fluorescence assay for pteroyl poly glutamates. Proceedings of the Australian Biochemical Society (11): 43, 1978

Effect of oral administration of folate sources on plasma folate levels in pigs: comparison between reduced and oxidized forms of folate. Canadian Journal of Animal Science 77(3): 497-502, 1997

Metabolism and physiological disposition of radioactively labeled folate derivatives in the rat. Biochemical Medicine 26(3): 435-450, 1981

Folate derived from cecal bacterial fermentation does not increase liver folate stores in 28-d folate-depleted male Sprague-Dawley rats. Journal of Nutrition 133(5): 1347-1354, 2003

Kinetics of folate turnover in pregnant women (second trimester) and nonpregnant controls during folic acid supplementation: stable-isotopic labeling of plasma folate, urinary folate and folate catabolites shows subtle effects of pregnancy on turnover of folate pools. Journal of Nutrition 131(7): 1928-1937, 2001

Further studies on the interaction of immobilized folate binding protein and enzyme folate conjugates an enzyme linked competitive binding assay for folate. Analytical Letters 19(15-16): 1653-1678, 1986