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The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers


Biochemical Journal 186(1): 235-242
The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers
About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labeled folate was incorporated into 2 folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, although less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0 nmol/g) and 10-formylpteroyltetraglutamate (0.25 nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase [glutamate carboxypeptidase, EC 3.4.12.10] treatment).


Accession: 006689561



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