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The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers



The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers



Biochemical Journal 186(1): 235-242



About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labeled folate was incorporated into 2 folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, although less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0 nmol/g) and 10-formylpteroyltetraglutamate (0.25 nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase [glutamate carboxypeptidase, EC 3.4.12.10] treatment).

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