+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers

The identification of the folate conjugates found in rat liver 48 hours after the administration of radioactively labeled folate tracers

Biochemical Journal 186(1): 235-242

About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labeled folate was incorporated into 2 folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, although less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0 nmol/g) and 10-formylpteroyltetraglutamate (0.25 nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase [glutamate carboxypeptidase, EC] treatment).

(PDF 0-2 workdays service: $29.90)

Accession: 006689561

Download citation: RISBibTeXText

Related references

The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers. Biochemical Journal 186(1): 235-242, 1980

Reduced 18F-Folate Conjugates as a New Class of PET Tracers for Folate Receptor Imaging. Bioconjugate Chemistry, 2018

Synthesis and in vitro/in vivo evaluation of 99mTc-labeled folate conjugates for folate receptor imaging. Nuclear Medicine and Biology 38(4): 557-565, 2011

Synthesis and evaluation of folate-bis and folate-CYCLAM conjugates for possible use as folate-receptor-targeted copper radiopharmaceuticals. Journal of Labelled Compounds & Radiopharmaceuticals 42(SUPPL 1): S821-S823, June, 1999

Impact of high and low folate diets on tissue folate receptor levels and antitumor responses toward folate-drug conjugates. Journal of Pharmacology and Experimental Therapeutics 327(3): 918-925, 2008

Liver folate conjugates related to nutritional folate sufficiency and a fluorescence assay for pteroyl poly glutamates. Proceedings of the Australian Biochemical Society (11): 43, 1978

Effect of oral administration of folate sources on plasma folate levels in pigs: comparison between reduced and oxidized forms of folate. Canadian Journal of Animal Science 77(3): 497-502, 1997

Metabolism and physiological disposition of radioactively labeled folate derivatives in the rat. Biochemical Medicine 26(3): 435-450, 1981

Folate derived from cecal bacterial fermentation does not increase liver folate stores in 28-d folate-depleted male Sprague-Dawley rats. Journal of Nutrition 133(5): 1347-1354, 2003

Kinetics of folate turnover in pregnant women (second trimester) and nonpregnant controls during folic acid supplementation: stable-isotopic labeling of plasma folate, urinary folate and folate catabolites shows subtle effects of pregnancy on turnover of folate pools. Journal of Nutrition 131(7): 1928-1937, 2001

Further Studies on the Interaction of Immobilized Folate Binding Protein and Enzyme-Folate Conjugates; An Enzyme-Linked Competitive Binding Assay for Folate. Analytical Letters 19(15-16): 1653-1678, 1986

Further studies on the interaction of immobilized folate binding protein and enzyme folate conjugates an enzyme linked competitive binding assay for folate. Analytical Letters 19(15-16): 1653-1678, 1986

Folate receptor specific anti-tumor activity of folate-mitomycin conjugates. Cancer ChemoTherapy and Pharmacology 58(2): 229-236, 2005

An unusual role of folate in the self-assembly of heparin-folate conjugates into nanoparticles. Nanoscale 7(37): 15185-15190, 2016

Development and preclinical evaluation of new (124)I-folate conjugates for PET imaging of folate receptor-positive tumors. Nuclear Medicine and Biology 41(6): 457-463, 2015