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The identity and properties of firefly luciferase inhibitors in urine






Analytical Biochemistry 114(2): 433-440

The identity and properties of firefly luciferase inhibitors in urine

The firefly luciferin-luciferase reagent may be used for the determination of low ATP. The lower limit of ATP measurements in body fluids [a means of detecting bacteriuria] is dependent upon the concentrations of inhibitors, e.g. Cl-, sulfate and urea. For urine and for a mixture of Cl-, sulfate and urea the peak light output following mixing of luciferase, luciferin and ATP, decreased exponentially with increasing inhibitor concentration. Peak light output increases linearly with increasing ATP concentration. A mathematical relationship between the exponential inhibitor response and the linear substrate response during codilution was established. This relationship predicted the existence of a dilution for each initial ATP-inhibitor mixture which would yield maximum light output. Such predictions agreed qualitatively with experimental observations when ATP-urine mixtures were diluted.

Accession: 006689626

PMID: 7304936

DOI: 10.1016/0003-2697(81)90508-x

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