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The induction of cyto kinin habituation in primary pith explants of tobacco nicotiana tabacum cultivar havana 425



The induction of cyto kinin habituation in primary pith explants of tobacco nicotiana tabacum cultivar havana 425



Planta (Heidelberg) 149(4): 402-407



Pith parenchyma tissue of N. tabacum L. cv. Havana 425 becomes cytokinin habituated when incubated at 35.degree. C on an auxin-containing medium. Under these conditions, habituated, hyperplastic nodules appear on the tissues. These nodules were used to estimate the incidence of habituation by a statistical method. The rate of habituation varied with the season. Tissue isolated from plants in the spring habituated .apprx. 7 times faster than did tissue isolated from plants in winter. The fact that the average rate, > 4 .times. 10-3 per cell generation, was 100-1000 times faster than the rate of somatic mutation in Nicotiana spp. and depended on the physiological state of the tissue provides further evidence that habituation involves epigenetic changes rather than rare, random genetic mutations. Kinetin (6-furfurylaminopurine) induced habituation; the concentration required depended on the duration of cytokinin treatment. For long incubation times, .apprx. 6 .times. 10-10 M kinetin, which is about 1000-fold lower than the concentration optimal for growth of cytokinin-requiring pith tissue, was sufficient to induce habituation. The habituated state is maintained by a positive feedback loop in which cytokinins either induce their own synthesis or inhibit their own degradation.

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Accession: 006694093

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