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The long term preservation of potato virus y and watermelon mosaic virus in liquid nitrogen in comparison to other preservation methods


, : The long term preservation of potato virus y and watermelon mosaic virus in liquid nitrogen in comparison to other preservation methods. Netherlands Journal of Plant Pathology 85(1): 23-30

Samples of watermelon mosaic virus (WMMV), consisting of clarified sap of diseased squash [Cucurbita pepo] plants, were highly infective after more than 4 yr of storage if the samples had been deep frozen at -18.degree. C, and lost more of their infectivity within 4 yr of preservation by freeze drying and subsequent storage of the samples at 4.degree. C. Potato virus Y (PVY) inocula, consisting of samples of clarified sap of diseased red peppers [Capsicum annuum], lost their infectivity in less than 4 yr if the samples had been deep frozen at -18.degree. C but retained their infectivity for more than 4 yr if the samples had been freeze dried and stored at 4.degree. C. A decrease in infectivity of both viruses in the deep frozen at -18.degree. C and freeze dried inocula could be observed even during the 1st months of storage. Samples of clarified sap stored in or over liquid N maintained their activity for at least 22 mo. for WMMV and 32 mo. for PVY with no indication of a decrease in infectivity of the viruses. Storage in or over liquid N seems therefore a promising long-term preservation method for plant viruses.

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