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The loss of phytochrome photo reversibility in vitro part 2 properties of killer and its reaction with phytochrome


, : The loss of phytochrome photo reversibility in vitro part 2 properties of killer and its reaction with phytochrome. Planta (Heidelberg) 135(3): 217-224

Killer, a substance extracted from stem tissue of etiolated pea seedlings (Pisum sativum L. v. Alaska), interacts specifically with the far-red-absorbing form of phytochrome (Pfr) in vitro in a temperature-independent, rapid, stoichiometric fashion to cause a loss of phytochrome photoreversibility. The chromatographic, solubility, and spectral properties of partially purified fractions indicate that Killer is a cyclic, unsaturated molecule containing ionizible hydroxyl groups; its MW is unknown, although probably low. Possible mechanisms by which the Killer-phytochrome interaction results in the loss of photoreversibility are discussed.

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