EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The low ionic strength reaction of human blood relationship between the binding of serum immuno globulin and complement to red blood cells and surface charge of the cells


, : The low ionic strength reaction of human blood relationship between the binding of serum immuno globulin and complement to red blood cells and surface charge of the cells. British Journal of Haematology 42(3): 403-416

Using the sucrose hemolysis reaction of Hartmann and Jenkins as a basic model, the low ionic strength reaction (LISR) of human blood was studied to determine serum Ig [immunoglobulin] uptake by RBC [red blood cells] with saline elution and 125I-IgG uptake, and complement fixation (CF) to RCB with lysis of PNH [paroxysmal nocturnal hemoglobinuria] cells and C3/C4 antiglobulin hemagglutination (AH) of normal cells. The saline eluates contained IgG and IgM and with traces of IgA. Their pH optima for the uptake by RBC were 6.0 .+-. 0.5, 5.5 .+-. 0.5 and approximately 5.0, respectively. The ratio of bound IgG to IgM was linearly related to the uptake pH. C4 AH and lysis were optimum at pH 6.0-7.5, whereas the maximum C3 AH was at pH 6.0 .+-. 0.5. The LISR performed at a constant pH (6.1 .+-. 0.1) showed that an increasing concentration of neuraminidase (VCN) used in pretreatment of RCB was associated with a decrease in IgG uptake and CF activity. A maximum VCN effect reduced the Ig uptake to approximately 20% of normal and abolished almost all the CF activity. An impaired LISR to various degrees was also observed with RBC pretreated with ficin, papain, bromelin, trypsin or protamine, and RCB from 2 individuals of En(a-) type. Preincubation of serum at LIS with and without RBC resulted in, respectively, a complete and partial consumption of C in the fluid phase. The latter was not enhanced or inhibited by the addition of VCN-treated RBC for preincubation. In the LISR the Ig uptake by RBC is an electrostatic interaction of the oppositely charged RBC and Ig and the CF to RBC results from C activation by the cell-bound IgG and IgM. A pH-dependent inactivation of the cell-bound C3 in the LISR was demonstrated.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006712224

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Oddvar F. Nygaard and Richard L. Potter, 1960: Effect of X-radiation on DNA metabolism in various tissues of the rat III Retention of labeled DNA in normal and irradiated animals. Radiation Research 12(2): 131-145

Wadsworth A.; Deroin J. P.; Rudant J. P., 1991: Imaging radar and offshore geology. International Geoscience and Remote Sensing Symposium 11: 2045-2046

Mannion A. M., 1986: Plant macrofossils and their significance in Quaternary palaeoecology; Part III, Applications; late glacial, postglacial and archaeological deposits. Progress in Physical Geography: , Pages 517-546. 1986.

Obermayer Straub, P.; Kayser, A.; Braun, S.; Loges, S.; Luettig, B.; Perheentupa, J.; Manns, M. P., 1998: LKM autoantibodies in autoimmune hepatitis, APS1 and chronic hepatitis C Quantification of anti-CYP2D6 autoantibodies with 35S-labeled recombinant protein and comparison of target proteins. Journal of Hepatology 28(SUPPL 1): 227

Ribalet B.; Beigelman P.M., 1981: Effects of divalent cations on beta cell electrical activity. The effects of various divalent cations, added to the external medium, on mouse .beta.-cell action potential were studied using intracellular microelectrodes. Changes in spike peak potential, as a function of external cation concentration, indicat...

O'farrell N.; Hoosen A.A.; Coetzee K.; Van Den Ende J., 1990: A rapid stain for the diagnosis of granuloma inguinale. Tissue smears were prepared from 55 men and eight women with genital ulceration using two staining techniques and examined by direct microscopy for the presence of Donovan bodies. Twenty three smears were positive using the May-Grunwald-Giemsa sta...

Gieseler, Frank; Dartsch, Dorothee C.; Stopper, Helga; Clark, Michael Robert; Bartscht, Tobias, 2002: Molecular events critical for apoptosis induction after topoisomerase II inhibitor treatment Proteolysis of topoisomerase II and defective DNA processing. Proceedings of the American Association for Cancer Research Annual Meeting 43: 387, March

Korchmaros I., 1968: Method for senile entropion operations abstract human. American Journal Of Ophthalmology: 936

Davoren, A., 1978: A survey of New Zealand peat resources. Data on climate, surface vegetation and land use, peat composition and the peat profile are provided for 59 peat swamps. Peatlands on Stewart and Chatham Islands and the region west of the main divide between Palmerston North and Wellington were n...

Neuhauser, T. S.; Derringer, G. A.; Thompson, L. D. R.; Fanburg Smith, J. C.; Aguilera, N. S. I.; Andriko, J. A.; Abbondanzo, S. L., 2000: Splenic inflammatory myofibroblastic tumor A clinicopathologic and immunophenotypic study of 12 cases. Laboratory Investigation 80(1): 14A, Jan