+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The low molecular weight autoinhibitor of sexual development in dictyostelium discoideum inhibits cell fusion and zygote differentiation

Canadian Journal of Biochemistry & Cell Biology 62(8): 722-731

The low molecular weight autoinhibitor of sexual development in dictyostelium discoideum inhibits cell fusion and zygote differentiation

During the sexual cycle of D. discoideum, zygote giant cell formation is regulated by an autoinhibitor. The autoinhibitor inhibits 2 events of zygote development: cell fusion and subsequent giant cell differentiation. The autoinhibitor is heat stable and has a MW around 500. Medium containing the autoinhibitor can be diluted 500-fold without loss of activity. Preliminary experiments show that, although levels of ammonia double during the 8-h period of autoinhibitor production, added ammonia does not mimic the inhibiting effect. cAMP at 1 mM inhibits both binucleate formation and differentiation, but the concentration of cAMP in 28-h cultures is only 13.4 pmol, a level which does not affect zygote development. Neither of these critical regulators of other developmental processes in D. discoideum is the autoinhibitor.

(PDF 0-2 workdays service: $29.90)

Accession: 006712235

Related references

Cell fusion, nuclear fusion, and zygote differentiation during sexual development of Dictyostelium discoideum. Developmental Biology 90(2): 375-382, 1982

Zygote giant cell differentiation in Dictyostelium discoideum: biochemical markers of specific stages of sexual development. Biochemistry and Cell Biology 70(10-11): 1200-1208, 1992

Chloroquine inhibits cell fusion during sexual development in dictyostelium discoideum. Canadian Journal of Microbiology 33(12): 1125-1129, 1987

Calcium calmodulin and the antagonistic action of an endogenous autoinhibitor of cell and pronuclear fusion in dictyostelium discoideum. O'day, D H (Ed ) Calcium As An Intracellular Messenger in Eucaryotic Microbes Xiii+418p American Society For Microbiology: Washington, D C , Usa Illus 392-410, 1990

The autoinhibitor of cell fusion in Dictyostelium inhibits calmodulin. Biochemical and Biophysical Research Communications 164(3): 1176-1181, 1989

Gamete fusion and cytokinesis preceding zygote establishment in the sexual process of Dictyostelium discoideum. Development, Growth & Differentiation 47(1): 25-35, 2005

Concanavalin a and wheat germ agglutinin binding glycoproteins associated with cell fusion and zygote differentiation in dictyostelium discoideum effects of calcium ions and tunicamycin on glycoprotein profiles. Biochemistry & Cell Biology 69(4): 282-290, 1991

Possible existence of a light-inducible protein that inhibits sexual cell fusion in Dictyostelium discoideum. Cell Structure and Function 16(2): 185-187, 1991

Ca2+ and cell fusion during sexual development in liquid cultures of Dictyostelium discoideum. Experimental Cell Research 126(2): 501-505, 1980

Molecular mechanism of sexual cell fusion in the cellular slime mold dictyostelium discoideum. Cell Structure & Function 12(6): 631, 1987