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The lupus erythematosus cell phenomenon new aspects in molecular biology and immuno cytology

, : The lupus erythematosus cell phenomenon new aspects in molecular biology and immuno cytology. Archives of Dermatological Research 266(2): 197-204

The effects of antinuclear antibodies on nuclear material can be observed directly in free [human] leukocyte nuclei in the supravital preparation according to Engel. Antinuclear antibodies prevent lysis of free nuclei; they obstruct normal degeneration of nuclei. The nuclear blockade is recognizable in the dark-clodded, rigid nuclei which remain small. These blocked nuclei are the visible expression of a development of complexes between chromatin and antinuclear antibodies. The protective effect of antinuclear antibodies apparently consists of obstruction of the DNases and histone-proteases bound to chromatin. Effects of antinuclear antibodies are in agreement with the structure of the chromatin nucleosomes. The nuclear blockade caused a persistence of antigenic material, which can create an increase of antibody production. The antinuclear blockade is an effect which sustains illness in vivo.

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