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The lympho toxin system in experimental animals part 2 physical and immunologic characteristics of molecules with lympho toxin activity rapidly released by murine lymphoid cells activated on lectin coated allogeneic mono layers in vitro

, : The lympho toxin system in experimental animals part 2 physical and immunologic characteristics of molecules with lympho toxin activity rapidly released by murine lymphoid cells activated on lectin coated allogeneic mono layers in vitro. Journal of Immunology 123(1): 317-324

The physicochemical and immunologic properties of cell-lytic molecules released in vitro by nonadherent C57/BL/6 splenocytes or nylon wool-enriched T [thymus-derived] cell populations activated on monolayers of PHA [phytohemagglutinin] coated [mouse fibroblast] L-929 cells were investigated. Cell-lytic molecules released by these lymphoid cells were physically heterogeneous. These molecules were separated by gel filtration into similar MW classes previously observed for human LT [lymphotoxin] molecules. Three major classes, termed complex (Cx) (> 200,000 d [daltons]), .alpha. heavy (.alpha.H) (110,000-140,000 d).alpha. light (.alpha.L) (60,000-90,000 d), and 2 minor classes, .beta., at 40,000-50,000 d and .gamma. at 10,000-20,000 d were observed. Chromatography of supernatants in high ionic strength buffers dissociated Cx and .alpha.H to the smaller MW .alpha.L form. Cx and .alpha.H MW classes are apparently physically related to the smaller MW .alpha.L class. Fractionation of the .alpha.H MW LT class by DEAE or PAGE [polyacrylamide gel electrophoresis] resolved these molecules into additional distinct subclasses. Antisera were made against fresh serum-free whole supernatants (anti-WS) or rechromatographed Ultrogel fractions containing .alpha.H molecules (anti-.alpha.H). Anti-.alpha.H and anti-WS react with all MW classes of murine LT molecules, indicating these various forms are immunologically related. These antisera did not react with LT molecules obtained from several other animal species or with nonspecific intracellular toxins, e.g., lysozomal enzymes, present in normal PMN [polymorphonuclear leukocyte] or phagocytic cells. Materials with cell-lytic activity present in these culture supernatants are apparently LT molecules, because: certain MW forms observed are similar to those reported previously, these various MW forms are all physically and immunologically interrelated. Murine LT molecules like human LT molecules are heterogeneous, but appear to comprise a system of subunits, in which the large MW form may dissociate into the smaller MW forms.

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Nistri A.; Constanti A., 1975: Effects of glutamate and glutamic acid di ethyl ester on the lobster muscle fiber and the frog spinal cord. European Journal of Pharmacology 31(2): 377-379

Ramos Elorduy De Conconi J.; Ramirez Martinez M., 1979: Effect of the dose rate of gamma rays on the oviposition and emergence of prostephanus truncatus coleoptera bostrychidae. An important pest of stored corn, P. truncatus (Horn.) was studied, applying 4 different dose rates of 15 krads to 10 day old females, using 2, 4, 8, and 12 Cobalt 60 sources of a .gamma.-beam apparatus. The number of females that died after 14 da...

Petty T.L.; Silvers G.W.; Baird M.D.; Stanford R.E.; Mitchell R.S., 1979: Small airway pathology is related to increased closing capacity in excised human lungs. American Review of Respiratory Disease 119(4 PART 2): 347

Clement, Omoshile O.; Puri, Swati; Gliubich, F.; Varma, Shikha; Freeman, Clive; Waldman, Marvin; Li, Jiabo, 2003: Comparing protein-bound ligand structures with in-silico genearted conformations. Abstracts of Papers American Chemical Society 226(1-2): CINF 56

Pinto, Donald, J.; Pitts, William, J.; Copeland, Robert, A.; Covington, Maryanne, B.; Trzaskos, James; Magolda, Ronald, 1995: Selective inhibition of cyclooxygenase-2: Diaryl heterocycles vs classical NSAIDS. This review discusses the in vitro and in vivo activities of selected anti-inflammatory diaryl heterocycles, as compared with selected classical NSAIDS. The diaryl heterocycle tiflamizole is a selective inhibitor of COX1 and shares the liabilities...

Pasenko, Michael, 2010: A skull and mandible of Mammut americanum Proboscidea, Mammalia from southeast Arizona. Journal of the Arizona-Nevada Academy of Science. Proceedings supplement 45

Chilian W.M.; Boatwright R.B.; Shoji T.; Griggs D.M.Jr, 1981: Evidence against significant resting sympathetic coronary vaso constrictor tone in the conscious dog. The primary objective of this study was to ascertain whether resting coronary blood flow is under tonic restraint due to sympathetically mediated .alpha.-adrenergic coronary vasoconstriction. To accomplish this, a technique for selectively sympath...

Aso, K; Yamashita, M; Arai, S; Fujimaki, M., 1974: Tryptophan-, threonine-, and lysine-enriched plasteins from zein. Agricultural and biological chemistry: 38 (3) 679-680

Lopez John A., 1990: Cyclic stratigraphy and the significance of resolution. AAPG Bulletin 74(5): 708

Wood, Horace Elmer, 1931: 2d Lower Oligocene Rhinoceroses of the genus Trigonias. The material, previously studied by Matthew and by Gregory and Cook, consists of numerous rhinoceros skeletons and skulls from the Lower Oligocene of Weld Co., Colorado. The first author regarded all the varying skulls as essentially one species o...