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The lysosomal enzyme binding receptor protein isolated from brain and its characteristics


, : The lysosomal enzyme binding receptor protein isolated from brain and its characteristics. Indian Journal of Biochemistry & Biophysics 25(1-2): 150-151

Lysosomal enzymes are high mannose glycoproteins and they are recognised through their mannose-6-phosphate residues by a receptor protein and carried to the lysosomal compartment. We have isolated from monkey brain the receptor protein by phosphomannan-Sepharose chromatography. The receptor protein is a high molecular weight aggregate as seen by gel electrophoresis under non-denaturing condition. On SDS-gel electrophoresis it shows the presence of several subunits. Alkaline phosphatase treatment or periodate treatment of brain lysosomal enzymes diminishes their binding to the receptor protein. The receptor protein immobilized on Sepharose could bind the brain lysosomal enzymes and be eluted either by mannose-6-phosphate (but not by several other sugars) or by citrate-phosphate buffer (pH 4.5). Alkaline phosphatase treatment of the enzymes did not prevent their binding to the receptor-Sepharose column but decreased the affinity of binding.

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Accession: 006712614

PMID: 3141263

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Panneerselvam, K.; Balasubramanian, A.S., 1989: Inhibition by lysosomal enzymes and mannose-6-phosphate of the phosphorylation of the lysosomal enzyme binding receptor protein from monkey brain. The lysosomal enzymes beta-glucuronidase and alpha-L-fucosidase and mannose-6-phosphate inhibited the phosphorylation of the lysosomal enzyme binding receptor protein prepared from monkey brain. Inhibition of both serine and tyrosine phosphorylati...

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