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The lysosomal enzyme binding receptor protein isolated from brain and its characteristics


Indian Journal of Biochemistry & Biophysics 25(1-2): 150-151
The lysosomal enzyme binding receptor protein isolated from brain and its characteristics
Lysosomal enzymes are high mannose glycoproteins and they are recognised through their mannose-6-phosphate residues by a receptor protein and carried to the lysosomal compartment. We have isolated from monkey brain the receptor protein by phosphomannan-Sepharose chromatography. The receptor protein is a high molecular weight aggregate as seen by gel electrophoresis under non-denaturing condition. On SDS-gel electrophoresis it shows the presence of several subunits. Alkaline phosphatase treatment or periodate treatment of brain lysosomal enzymes diminishes their binding to the receptor protein. The receptor protein immobilized on Sepharose could bind the brain lysosomal enzymes and be eluted either by mannose-6-phosphate (but not by several other sugars) or by citrate-phosphate buffer (pH 4.5). Alkaline phosphatase treatment of the enzymes did not prevent their binding to the receptor-Sepharose column but decreased the affinity of binding.


Accession: 006712614

PMID: 3141263



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